Clement T, Visseaux-Coletto B, Adnet J J, Ploton D, Lardennois B
Service d'Urologie, CHU Robert Debré, Reims.
Prog Urol. 1991 Apr;1(2):216-29.
Nucleolar Organisers (NORs) are intranucleolar segments of DNA coding for ribosomal RNA. The agyrophilic proteins (AgNOR) associated with NORs allow them to be cytolabelled on paraffin sections. The number of NORs (NOR index) is correlated with cellular proliferation and has a diagnostic and prognostic value in neoplastic disease. The AgNOR method was analysed in a series of 37 superficial bladder tumours with different clinical courses. The NORs were counted in normal urothelium, on superficial tumours used to establish the diagnosis of the disease and on recurrent superficial tumours. The NOR index was 4.54 for normal urothelium, 5.89 for non-invasive superficial tumours, 7.33 for invasive superficial tumours, and 9.75 for invasive recurrences. These results demonstrate an increase in nucleolar argyrophilia with invasion and invasive potential of superficial bladder tumours which were initially homogeneous for stage and grade. The AfNOR method could constitute a new method of early histoprognostic evaluation for urothelial tumours.
核仁组织者区（NORs）是编码核糖体RNA的DNA的核仁内片段。与NORs相关的嗜银蛋白（AgNOR）使它们能够在石蜡切片上进行细胞标记。NORs的数量（NOR指数）与细胞增殖相关，在肿瘤性疾病中具有诊断和预后价值。对一系列37例具有不同临床病程的浅表性膀胱肿瘤进行了AgNOR方法分析。在正常尿路上皮、用于确诊疾病的浅表肿瘤以及复发性浅表肿瘤中对NORs进行计数。正常尿路上皮的NOR指数为4.54，非侵袭性浅表肿瘤为5.89，侵袭性浅表肿瘤为7.33，侵袭性复发肿瘤为9.75。这些结果表明，对于最初在分期和分级上较为一致的浅表性膀胱肿瘤，随着侵袭和侵袭潜能的增加，核仁嗜银性增强。AgNOR方法可构成一种用于尿路上皮肿瘤早期组织学预后评估的新方法。
