Magalhães J B, Andrade S G
Centro de Pesquisas Gonçalo Moniz/Fundação Oswaldo Cruz, Universidade Federal da Bahia, Salvador, BA.
Rev Soc Bras Med Trop. 1991 Oct-Dec;24(4):209-16. doi: 10.1590/s0037-86821991000400002.
To study the influence of the intermediate host stage on the course of mouse infection, Trypanosoma cruzi belonging to the Peruvian (Type I), 12 SF (Type II) and Colombian (Type III) strains were passaged through either Rhodnius prolixus, Panstrongylus megistus or Triatoma infestans. T. cruzi metacyclic forms (dose 10(4)) from the different strains were obtained from each bug and inoculated into 8-10 gm mice. Comparison was made in mice inoculated with blood forms. Parasitaemia curves were plotted in the peripheral blood for each strain, reaching more elevated peaks with Peruvian strain parasites from P. megistus and R. prolixus, 12 SF strain from P. megistus and Colombian strain from R. prolixus. Tissue tropism and histopathological patterns were those usually seen in mice infected with each respective strain type. Peruvian virulence was the same for all groups. Slender forms predominate among mice inoculated with metacyclic forms of Colombian and 12 SF strains, probably an adaptative parasite change related to the intermediate host.
为研究中间宿主阶段对小鼠感染过程的影响，将属于秘鲁株（I型）、12 SF株（II型）和哥伦比亚株（III型）的克氏锥虫分别通过长红猎蝽、巨蝽或大锥蝽进行传代。从每种虫体获得不同株系的克氏锥虫循环后期形态（剂量为10⁴），并接种到8 - 10克重的小鼠体内。对接种血液形态虫体的小鼠进行了比较。绘制了各株系外周血中的寄生虫血症曲线，来自巨蝽和长红猎蝽的秘鲁株寄生虫、来自巨蝽的12 SF株以及来自长红猎蝽的哥伦比亚株达到了更高的峰值。组织嗜性和组织病理学模式是各相应株系感染小鼠中常见的情况。所有组的秘鲁株毒力相同。在接种哥伦比亚株和12 SF株循环后期形态的小鼠中，细长型为主，这可能是与中间宿主相关的寄生虫适应性变化。
