Dittrich Marcus, Birschmann Ingvild, Mietner Silke, Sickmann Albert, Walter Ulrich, Dandekar Thomas
Department of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, Würzburg D-97074, Germany.
Arterioscler Thromb Vasc Biol. 2008 Jul;28(7):1326-31. doi: 10.1161/ATVBAHA.107.161000. Epub 2008 May 1.
Assembly of a comprehensive proteome and transcriptome database of human platelets, derivation of a model of the platelet-specific interactome, and generation of a functional interaction map of platelet phosphorylations and kinases.
Interactions are derived from literature-curated data from HPRD and yeast two hybrid (Y2H) and mapped to platelet-specific expression data (SAGE or proteome). From this a cell-type specific model of platelet proteins and protein-protein interactions is derived. The obtained inventory of platelet-specific proteins includes key domains, protein GO annotations, and receptors. Collected interactions point to new platelet signaling components, actin remodeling processes, and pharmacological targets and offer incentives for further studies (eg, on the IPP complex). Integration of platelet-specific phosphoproteins and the characterization of the platelet kinase repertoire sketch a first outline of kinase signaling in human platelets.
A first view of the platelet interactome, platelet phosphorylation, and platelet kinome is available from the in silico data.
构建人类血小板的综合蛋白质组和转录组数据库，推导血小板特异性相互作用组模型，并生成血小板磷酸化和激酶的功能相互作用图谱。
相互作用源自HPRD的文献整理数据和酵母双杂交（Y2H），并映射到血小板特异性表达数据（SAGE或蛋白质组）。由此推导出血小板蛋白质和蛋白质-蛋白质相互作用的细胞类型特异性模型。所获得的血小板特异性蛋白质清单包括关键结构域、蛋白质GO注释和受体。收集到的相互作用指向新的血小板信号成分、肌动蛋白重塑过程和药理学靶点，并为进一步研究（如IPP复合物）提供了动力。血小板特异性磷酸化蛋白质的整合以及血小板激酶库的表征勾勒出了人类血小板激酶信号的初步轮廓。
从计算机数据中可以初步了解血小板相互作用组、血小板磷酸化和血小板激酶组。
