[液相色谱/串联质谱法（LC/MS/MS）快速测定减肥保健食品中的药用成分]

[Rapid determination of medical components found in the health food for weight loss by liquid chromatography/tandem mass spectrometry (LC/MS/MS)].

作者信息

Date Hideyo, Toyota Akie, Terauchi Masahiro, Sugimura Mitsunori, Matsuo Takeshi, Mochiike Chieko

机构信息

Hiroshima Prefectural Technology Research Institute, Health and Environment Center, Minami-ku, Hiroshima, Japan.

出版信息

Yakugaku Zasshi. 2008 May;128(5):811-7. doi: 10.1248/yakushi.128.811.

DOI:10.1248/yakushi.128.811

PMID:18451630

Abstract

A method for the rapid determination of 11 medical components found in health foods for weight loss using liquid chromatography/tandem mass spectrometry (LC/MS/MS) has been developed. HPLC separation is performed on an ODS column with the gradient elution method. The mobile phase consists of two solvents. Solvent A is water and solvent B is methanol/acetonitrile (1:1), and both contain 0.1% formic acid and 5 mmol/l of ammonium acetate. Each medical component is analyzed with multiple-reaction monitoring (MRM) in both negative and positive modes through electrospray ionization (ESI). The recovery rates of the 11 medical components added to commercially available health foods were 46.3-114% and each coefficient of variation was 13.7% or less. It was confirmed that this method is applicable to the urgent analysis of health foods that have caused damage to health.

摘要

已开发出一种使用液相色谱/串联质谱法（LC/MS/MS）快速测定减肥健康食品中11种药用成分的方法。采用梯度洗脱法在ODS柱上进行高效液相色谱分离。流动相由两种溶剂组成。溶剂A为水，溶剂B为甲醇/乙腈（1:1），两者均含有0.1%甲酸和5 mmol/L乙酸铵。通过电喷雾电离（ESI），以多反应监测（MRM）模式在负离子和正离子模式下对每种药用成分进行分析。添加到市售健康食品中的11种药用成分的回收率为46.3% - 114%，各变异系数均为13.7%或更低。证实该方法适用于对已造成健康损害的健康食品进行紧急分析。