Wang Wei, Feng Shun, Li Ya-ni, Wu Meiying, Wang Jide
Key Laboratory of Oil & Gas Fine Chemicals, Ministry of Education, Urumqi 830046, China.
J Chromatogr A. 2008 Jun 6;1193(1-2):142-5. doi: 10.1016/j.chroma.2008.04.006. Epub 2008 Apr 9.
Dioxygen complexes play important roles in organisms' bodies, so the determination of binding-dioxygen has practical significance. A simple and robust method based on headspace gas chromatography was proposed to determine the binding-dioxygen in dioxygen complexes. By measuring the content change of nitrogen gas in a vial, the amount of oxygen released from dixoygen complexes can be determined. The method was validated using potassium chlorate as model sample, and the results exhibited good recoveries (90-99%) with the relative standard deviation less than 8%. It was also used to analyze dioxygen complex of cobalt bis(salicylaldehyde) ethylenediimine and polyamine cobalt complexes prepared by solid-phase reaction.
双氧络合物在生物体中发挥着重要作用，因此测定结合双氧具有实际意义。提出了一种基于顶空气相色谱的简单且可靠的方法来测定双氧络合物中的结合双氧。通过测量小瓶中氮气的含量变化，可以确定双氧络合物释放出的氧气量。该方法以氯酸钾作为模型样品进行了验证，结果显示回收率良好（90 - 99%），相对标准偏差小于8%。它还被用于分析双（水杨醛）乙二胺钴双氧络合物以及通过固相反应制备的多胺钴络合物。
