对11至16岁儿童全唾液和龈沟液中胱抑素C、白细胞介素-1β和肿瘤坏死因子-α水平的评估。

The evaluation of cystatin C, IL-1beta, and TNF-alpha levels in total saliva and gingival crevicular fluid from 11- to 16-year-old children.

作者信息

Ulker A Evren, Tulunoglu Ozlem, Ozmeric Nurdan, Can Murat, Demirtas Selda

机构信息

Department of Pediatric Dentistry, Faculty of Dentistry, Gazi University, Ankara, Turkey.

出版信息

J Periodontol. 2008 May;79(5):854-60. doi: 10.1902/jop.2008.070422.

DOI:10.1902/jop.2008.070422

PMID:18454664

Abstract

BACKGROUND

The aim of this study was to evaluate the levels of cystatin C, interleukin-1beta (IL-1beta), and tumor necrosis factor-alpha (TNF-alpha) in the total saliva and gingival crevicular fluid (GCF) of periodontally healthy children (PHC) and children with gingivitis (CG) who were between 11 and 16 years old.

METHODS

The study was carried out with 10 PHC and 25 CG. Unstimulated total saliva and GCF samples were obtained. Clinical parameters, including probing depth (PD), clinical attachment loss (CAL), plaque index (PI), gingival index (GI), and gingival bleeding index (GBI), were assessed. GCF samples were collected from four maxillary upper incisors. After sampling, biochemical analyses were performed using latex particle-enhanced turbidimetric immunoassay for cystatin C and enzyme-linked immunosorbent assay for IL-1beta and TNF-alpha. The multivariate analysis of variance test was used for statistical evaluation.

RESULTS

In total saliva, cystatin C and TNF-alpha levels were higher in PHC, and IL-1beta levels were higher in CG, but the differences were not statistically significant. In GCF, cystatin C levels were higher in PHC (P >0.05), whereas TNF-alpha and IL-1beta levels were higher in CG (P >0.05). In the CG group, there were positive correlations between the GCF cystatin C level and the PI of the sampled site (r = 0.488; P <0.05); also, GCF IL-1beta (r = 0.603; P <0.05) and TNF-alpha (r = 0.456; P <0.05) levels were positively correlated with PD and CAL. For the whole mouth and the sampled sites, PI, GI, GBI, PD, and CAL values were higher in CG (P <0.05), but no significant differences were detected between GCF volumes of the two groups.

CONCLUSIONS

To the best of our knowledge, this study represents the first evaluation of cystatin C in the gingival disease mechanism in children. Our results showed that total saliva and GCF cystatin C levels were higher in PHC (P >0.05), but there was no correlation between cystatin C levels and IL-1beta or TNF-alpha levels in total saliva or GCF.

摘要

背景

本研究旨在评估11至16岁牙周健康儿童（PHC）和牙龈炎儿童（CG）的全唾液和龈沟液（GCF）中胱抑素C、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的水平。

方法

该研究纳入了10名PHC儿童和25名CG儿童。采集非刺激性全唾液和GCF样本。评估临床参数，包括探诊深度（PD）、临床附着丧失（CAL）、菌斑指数（PI）、牙龈指数（GI）和牙龈出血指数（GBI）。从四颗上颌中切牙采集GCF样本。采样后，采用乳胶颗粒增强比浊免疫分析法检测胱抑素C，采用酶联免疫吸附测定法检测IL-1β和TNF-α。采用多因素方差分析进行统计学评估。

结果

在全唾液中，PHC儿童的胱抑素C和TNF-α水平较高，CG儿童的IL-1β水平较高，但差异无统计学意义。在GCF中，PHC儿童的胱抑素C水平较高（P>0.05），而CG儿童的TNF-α和IL-1β水平较高（P>0.05）。在CG组中，GCF胱抑素C水平与采样部位的PI呈正相关（r=0.488；P<0.05）；此外，GCF IL-1β（r=0.603；P<0.05）和TNF-α（r=0.456；P<0.05）水平与PD和CAL呈正相关。对于全口和采样部位，CG儿童的PI、GI、GBI、PD和CAL值较高（P<0.05），但两组GCF体积之间未检测到显著差异。