南非鲍鱼单核苷酸多态性（SNP）的发现与评估：一种靶向EST方法。
Discovery and evaluation of single nucleotide polymorphisms (SNPs) for Haliotis midae: a targeted EST approach.
作者信息
Bester A E, Roodt-Wilding R, Whitaker H A
机构信息
Animal Molecular Genetics Laboratory, Department of Genetics, Stellenbosch University, Stellenbosch, South Africa.
出版信息
Anim Genet. 2008 Jun;39(3):321-4. doi: 10.1111/j.1365-2052.2008.01728.x.
In this study, we describe the first set of SNP markers for the South African abalone, Haliotis midae. A cDNA library was constructed from which ESTs were selected for the screening of SNPs. The observed frequency of SNPs in this species was estimated at one every 185 bp. When characterized in wild-caught abalone, the minor allele frequencies and F(ST) estimates for every SNP indicated that these markers may potentially be useful for population analysis, parentage assignment and linkage mapping in Haliotis midae. No linkage disequilibrium was observed between SNPs originating from different EST sequences. These SNPs, together with additional SNPs currently being developed, will provide a useful complementary set of markers to the currently available genetic markers in abalone.
在本研究中，我们描述了南非鲍鱼（Haliotis midae）的第一组单核苷酸多态性（SNP）标记。构建了一个cDNA文库，从中选择了表达序列标签（EST）用于SNP筛选。该物种中SNP的观察频率估计为每185个碱基对出现一个。在对野生捕获的鲍鱼进行特征分析时，每个SNP的次要等位基因频率和F(ST)估计值表明，这些标记可能对南非鲍鱼的种群分析、亲权鉴定和连锁图谱构建有用。未观察到源自不同EST序列的SNP之间存在连锁不平衡。这些SNP，连同目前正在开发的其他SNP，将为鲍鱼目前可用的遗传标记提供一组有用的互补标记。
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