用于基因组图谱构建的太平洋鲍基因靶向 SNP 标记的开发 **解析**： - 原文中“Pacific abalone”直译为“太平洋鲍”，但考虑到中文的表达习惯，通常将其译为“太平洋鲍”或“太平洋牡蛎”。 - “Haliotis discus hannai Ino.”是太平洋鲍的学名，原文保留了英文原文。
Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino.
机构信息
Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China.
出版信息
Mol Biol Rep. 2010 Dec;37(8):3779-84. doi: 10.1007/s11033-010-0032-3. Epub 2010 Mar 10.
Useful and novel DNA markers are needed for aquaculture genetics and breeding. In this study, we report the discovery and development of gene-targeted single nucleotide polymorphisms (SNPs) for genomic mapping in the Pacific abalone Haliotis discus hannai Ino. Single EST or EST-contigs from 66 genes that had positive BLASTx matches (E-value ≤ 1e-8) were used for polymerase chain reaction (PCR) amplification. PCR products from the two parents of one mapping family were directly sequenced, and 83 SNP loci were found from 17 genes. Allele-specific PCR (AS-PCR) was developed and optimized for genotyping of 11 SNP loci in 120 progeny of the mapping family. Nine of the loci conformed to the expected Mendelian ratio of 1:1 based on the χ(2) test (P > 0.05) and could potentially be used for linkage map construction. Our data also indicate that the sequencing of two parents may be a practical strategy for the discovery of informative SNPs for linkage mapping in a particular mapping population.
需要有用且新颖的 DNA 标记来进行水产养殖遗传学和育种。在这项研究中，我们报告了太平洋鲍 Haliotis discus hannai Ino 基因组图谱基因靶向单核苷酸多态性（SNP）的发现和发展。使用来自 66 个基因的正向 BLASTx 匹配（E 值≤1e-8）的单 EST 或 EST 连续序列进行聚合酶链反应（PCR）扩增。对一个图谱家系的两个亲本的 PCR 产物进行直接测序，从 17 个基因中发现了 83 个 SNP 位点。针对 120 个图谱家系后代的 17 个基因中的 11 个 SNP 位点开发并优化了等位基因特异性 PCR（AS-PCR）用于基因型分析。9 个位点基于 χ(2)检验（P > 0.05）符合预期的 1:1 孟德尔分离比，可能可用于连锁图谱构建。我们的数据还表明，对两个亲本进行测序可能是在特定图谱群体中发现用于连锁图谱的信息性 SNP 的一种实用策略。
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