Worrall S, De Jersey J, Shanley B C, Wilce P A
Department of Biochemistry, University of Queensland, Australia.
Alcohol Alcohol Suppl. 1991;1:255-60.
Rats fed ethanol for periods of 3-27 months were found to produce an immune response to acetaldehyde-modified proteins, whereas treatment for a shorter period (3 weeks) did not lead to such a response. The antibodies from the ethanol-fed animals were shown to be reactive with a series of proteins modified by acetaldehyde in vitro. This suggests that reactivity towards the modified proteins is independent of the carrier protein, with antibodies being raised which interact solely with the acetaldehyde-containing adduct and not the protein itself. We have also been able to demonstrate the presence of modified proteins in the liver cytosol of these ethanol-fed rats. However, unlike previous reports which suggested that only one protein was modified, we found that many cytosolic proteins were modified. This suggests that the modification of proteins by acetaldehyde is less specific than was previously thought.
研究发现，喂食乙醇3至27个月的大鼠会对乙醛修饰的蛋白质产生免疫反应，而较短时间（3周）的处理则不会引发这种反应。来自喂食乙醇动物的抗体被证明能与一系列体外被乙醛修饰的蛋白质发生反应。这表明对修饰蛋白质的反应性与载体蛋白无关，所产生的抗体仅与含乙醛的加合物相互作用，而不与蛋白质本身相互作用。我们还能够证明这些喂食乙醇的大鼠的肝细胞溶质中存在修饰蛋白质。然而，与之前认为只有一种蛋白质被修饰的报道不同，我们发现许多细胞溶质蛋白都被修饰了。这表明乙醛对蛋白质的修饰比之前认为的特异性更低。
