以功能化硫化镉纳米粒子为荧光探针测定环丙沙星
Determination of ciprofloxacin with functionalized cadmium sulfide nanoparticles as a fluorescence probe.
作者信息
Li Dan, Yan Zheng-Yu, Cheng Wei-Qing
机构信息
Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2008 Dec 15;71(4):1204-11. doi: 10.1016/j.saa.2008.03.024. Epub 2008 Mar 27.
A novel assay of ciprofloxacin with a sensitivity at the microgram level is proposed based on the measurement of enhanced fluorescence intensity signals resulting from the interaction of functionalized nano-CdS with ciprofloxacin. The CdS nanoparticles was synthesized by thioacetamide (TAA) and cadmium nitrate (Cd(NO(3))(2)) in the alkaline solution. At pH 7.4, the fluorescence signals of functionalized nano-CdS were greatly enhanced by ciprofloxacin with the increase concentration of ciprofloxacin. Linear relationship can be established between the enhanced fluorescence intensity and ciprofloxacin concentration in the range of (1.25-8.75)x10(-4) mg mL(-1) ((3.77-26.4) x 10(-4)mmol L(-1)) or (8.75-1200) x 10(-4)mg mL(-1) ((26.4-3625) x 10(-4) mmol L(-1)). The limit of detection is 7.64 x 10(-6) mg mL(-1) (2.31 x 10(-5)mmol L(-1)). Based on this, a new direct quantitative determination method for ciprofloxacin in human serum samples without separation of foreign substances was established. The contents of ciprofloxacin in human serum samples were determined with recovery of 95-105% and relative standard deviation (R.S.D.) of 1.5-2.5%. This method was proved to be very sensitive, rapid, simple and tolerance of most interfering substances.
基于功能化纳米硫化镉与环丙沙星相互作用产生的增强荧光强度信号的测量，提出了一种灵敏度达微克级的新型环丙沙星检测方法。硫化镉纳米颗粒由硫代乙酰胺（TAA）和硝酸镉（Cd(NO₃)₂）在碱性溶液中合成。在pH 7.4条件下，随着环丙沙星浓度的增加，功能化纳米硫化镉的荧光信号显著增强。在(1.25 - 8.75)×10⁻⁴ mg mL⁻¹（(3.77 - 26.4)×10⁻⁴ mmol L⁻¹）或(8.75 - 1200)×10⁻⁴ mg mL⁻¹（(26.4 - 3625)×10⁻⁴ mmol L⁻¹）范围内，增强的荧光强度与环丙沙星浓度之间可建立线性关系。检测限为7.64×10⁻⁶ mg mL⁻¹（2.31×10⁻⁵ mmol L⁻¹）。基于此，建立了一种无需分离杂质即可直接定量测定人血清中环丙沙星的新方法。测定了人血清样品中环丙沙星的含量，回收率为95% - 105%，相对标准偏差（R.S.D.）为1.5% - 2.5%。该方法被证明非常灵敏、快速、简单且对大多数干扰物质具有耐受性。
Zotero 插件