Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, No. 1 Renmin Road, Wuhu 241000, PR China.
Talanta. 2010 Apr 15;81(1-2):438-43. doi: 10.1016/j.talanta.2009.12.021. Epub 2009 Dec 21.
Glutathione (GSH)-capped CdS nanoparticles was successfully synthesized in aqueous solutions at room temperature. Based on the characteristics of synchronous fluorescence spectroscopy (SFS), a simple method with high sensitivity and selectivity was developed for determination of Hg(2+) with GSH-capped CdS nanoparticles as a fluorescence probe. The maximum synchronous fluorescence is located at 335 nm, scanning with excitation and emission wavelengths of 260 and 440 nm (Delta lambda=lambda(em)-lambda(ex)=180 nm), respectively. Under optimal conditions, the quenched fluorescence intensity increased linearly with the concentration of Hg(2+) ranging from 0.15 x 10(-7) to 125 x 10(-7) mol L(-1). The limit of detection was 4.5 x 10(-9) mol L(-1). Compared with several fluorescence methods, the proposed method exhibited several merits in terms of its linear range and the sensitivity. The quenching mechanism was also discussed. Moreover, analytical application of the method was demonstrated by water samples.
谷胱甘肽（GSH）包覆的 CdS 纳米粒子在室温的水溶液中成功合成。基于同步荧光光谱（SFS）的特点，提出了一种以 GSH 包覆的 CdS 纳米粒子作为荧光探针，用于测定 Hg（2+）的高灵敏度和选择性的简单方法。最大同步荧光位于 335nm，分别以激发和发射波长 260nm 和 440nm 进行扫描（Delta lambda=lambda(em)-lambda(ex)=180nm）。在最佳条件下，Hg（2+）的浓度在 0.15×10（-7）到 125×10（-7）mol L（-1）范围内，猝灭荧光强度线性增加。检测限为 4.5×10（-9）mol L（-1）。与几种荧光方法相比，该方法在其线性范围和灵敏度方面具有几个优点。还讨论了猝灭机制。此外，还通过水样验证了该方法的分析应用。
