Prajapati Santosh Kumar, Tripathi B D
Pollution Ecology Research Laboratory, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, India.
Environ Int. 2008 Nov;34(8):1092-6. doi: 10.1016/j.envint.2008.03.009. Epub 2008 May 2.
In the present study Tradescantia micronucleus (Trad-MCN) bioassay was performed to assess the genotoxicity of air pollutants in Varanasi City. The experiment was performed during October 2006 to April 2007. For Tradescantia micronucleus (Trad-MCN) bioassay four sites were selected, three in the city having different traffic characteristics and one control site virtually free from traffic intervention. Twenty young Tradescantia pallida inflorescences were collected from each sampling site during the study period and micronuclei frequencies were determined in early tetrads of pollen mother cells and expressed as MCN/100 tetrads. During the same period the concentration of different air pollutants were also measured. Tradescantia micronucleus (Trad-MCN) bioassay showed that the plants kept in areas having higher traffic emissions evidence higher micronuclei frequencies than samples kept at control site. The study indicates that in situ biomonitoring using higher plants may be useful for characterizing genotoxic air pollutants in areas even without any sophisticated instrument.
在本研究中，进行了紫露草微核（Trad-MCN）生物测定，以评估瓦拉纳西市空气污染物的遗传毒性。实验于2006年10月至2007年4月期间进行。对于紫露草微核（Trad-MCN）生物测定，选择了四个地点，其中三个在城市中，具有不同的交通特征，一个对照地点几乎没有交通干扰。在研究期间，从每个采样地点收集了20个年轻的紫露草花序，并在花粉母细胞的早期四分体中测定微核频率，并表示为MCN/100个四分体。在同一时期，还测量了不同空气污染物的浓度。紫露草微核（Trad-MCN）生物测定表明，与保存在对照地点的样本相比，保存在交通排放较高地区的植物微核频率更高。该研究表明，即使没有任何精密仪器，使用高等植物进行原位生物监测也可能有助于表征某地区的遗传毒性空气污染物。
