Department of Pathology, School of Medicine, University of Sao Paulo - Sao Paulo, Sao Paulo, Brazil; Study Group on Urban Agriculture, Institute of Advanced Studies, University of Sao Paulo (IEA), Brazil.
Department of Pathology, School of Medicine, University of Sao Paulo - Sao Paulo, Sao Paulo, Brazil.
Sci Total Environ. 2017 Jan 1;575:1438-1444. doi: 10.1016/j.scitotenv.2016.09.221. Epub 2016 Oct 5.
Urban gardening is a growing global phenomenon with a positive impact on society. Despite several associated benefits, growing vegetables in urban gardens that are localized in highly polluted areas poses questions about the safety of the produced food. Therefore, the identification of risk factors that result in possible deleterious effects to human health is important for realizing all of the benefits to society. We evaluated the use of two biomonitoring methods in ten urban gardens of Sao Paulo city and one control site: the micronuclei frequencies for early tetrads of Tradescantia pallida (Rose) Hunt. cv. "Purpurea" Boom (hereafter, Trad-MCN) as a short-term indicator of genotoxic response and tree barks to quantify the accumulation of traffic-related chemical elements as a long-term biomarker of air pollution in urban gardens. Mature plants of Tradescantia pallida were exposed in each garden, and their inflorescences were sampled over three months. A random set of 300 early tetrads in 13 to 21 slides per garden were evaluated for micronuclei frequencies. Elemental concentrations in 428 tree barks samples from 107 different trees in the areas surrounding urban gardens were quantified using an energy dispersive X-ray fluorescence spectrometer. The frequency of Trad-MCN has a significant correlation with traffic variables and chemical elements related to road dust and tailpipe emissions deposited in tree barks. Negative associations between Trad-MCN and both the distance through traffic and the presence of vertical obstacles were observed in the community gardens. The Mn/Zn concentrations in tree barks were associated with increased Trad-MCN.
城市园艺是一种全球范围内日益增长的现象，对社会具有积极影响。尽管存在一些相关益处，但在高度污染地区本地化的城市花园中种植蔬菜，会引发对所产食品安全性的质疑。因此，确定导致对人类健康产生有害影响的风险因素，对于实现社会的全部益处非常重要。我们评估了两种生物监测方法在圣保罗市的 10 个城市花园和一个对照点中的使用情况：白花紫露草早期四分体的微核频率（Rose）亨特。cv。“Purpurea”繁荣（以下简称Trad-MCN）作为遗传毒性反应的短期指标，以及树木树皮以量化与交通有关的化学元素在城市花园中的积累作为空气污染的长期生物标志物。在每个花园中都暴露了成熟的白花紫露草植物，并在三个月的时间内对其花序进行了采样。对每个花园的 13 到 21 张幻灯片中的 300 个早期四分体进行微核频率评估。使用能量色散 X 射线荧光光谱仪对来自城市花园周围 107 棵不同树木的 428 个树皮样本中的元素浓度进行定量分析。Trad-MCN 的频率与交通变量以及与道路灰尘和排气管排放物相关的化学元素在树木树皮中的沉积具有显著相关性。在社区花园中，Trad-MCN 与交通流量和垂直障碍物的存在之间呈负相关。树木树皮中的 Mn/Zn 浓度与 Trad-MCN 的增加有关。
