Verma Vikas, Chakravarti Anita, Kar Premashis
Department of Microbiology, Maulana Azad Medical College and Associated Lok Nayak Hospitals, Bahadur Shah Zafar Marg, New Delhi 110002, India.
Diagn Microbiol Infect Dis. 2008 Aug;61(4):408-14. doi: 10.1016/j.diagmicrobio.2008.03.011. Epub 2008 May 2.
The characterization of hepatitis C virus (HCV) strains has important prognostic, therapeutic, and epidemiologic implications. The present study was intended to determine the HCV genotypes from Northern India in chronic liver disease (CLD) patients. The response to therapy in various subtypes was also evaluated in patients with chronic hepatitis. Furthermore, the genotypes were correlated with the biochemical and histopathologic parameters to assess the severity of liver disease. Five milliliter of blood sample was collected aseptically from 98 patients with CLD due to hepatitis C. Genotyping was performed by restriction fragment length polymorphism and type-specific polymerase chain reaction, followed by direct sequencing. The HCV genotypes depicted were 1a (6.12%), 1b (8.16%), 1c (8.16%), 3a (17.34%), 3b (6.12%), 3a + 3b (40.81%), 3a + 1b (7.14%), and an unclassified type of 6.12%. The present study revealed that HCV genotype 3 is the predominant one, with 40.81% (40/98) being a mixed type of 3a with 3b and identification of newer subtype 1c. HCV subtypes 1a, 3a, and 3b are responding to therapy, whereas 1b (40%, 2/5), 1c (66.67%, 2/3), 3g (33.3%, 1/3), 3a + 3b (41.6%, 5/12), and mixed type 3a + 1b (66.67%, 4/6) are poor responders to therapy. The biochemical profiles were higher in mixed type followed by types 3 and 1, whereas the histologic parameters revealed that the severity of liver disease was more in mixed type followed by types 1 and 3, which also showed poor response to therapy.
丙型肝炎病毒（HCV）毒株的特征具有重要的预后、治疗和流行病学意义。本研究旨在确定印度北部慢性肝病（CLD）患者的HCV基因型。还评估了慢性肝炎患者对各种亚型治疗的反应。此外，将基因型与生化和组织病理学参数相关联，以评估肝病的严重程度。从98例丙型肝炎所致的CLD患者中无菌采集5毫升血样。通过限制性片段长度多态性和型特异性聚合酶链反应进行基因分型，随后进行直接测序。所描绘的HCV基因型为1a（6.12%）、1b（8.16%）、1c（8.16%）、3a（17.34%）、3b（6.12%）、3a + 3b（40.81%）、3a + 1b（7.14%），以及未分类类型占6.12%。本研究显示，HCV基因型3是主要类型，40.81%（40/98）为3a与3b的混合型，并鉴定出较新的亚型1c。HCV亚型1a、3a和3b对治疗有反应，而1b（40%，2/5）、1c（66.67%，2/3）、3g（33.3%，1/3）、3a + 3b（41.6%，5/12）和混合型3a + 1b（66.67%，4/6）对治疗反应较差。混合型的生化指标较高，其次是3型和1型，而组织学参数显示，混合型肝病的严重程度更高，其次是1型和3型，这也显示出对治疗反应较差。
