Laughlin Elsa, Burke George, Pugliese Alberto, Falk Ben, Nepom Gerald
Benaroya Research Institute at Virginia Mason, 1201 Ninth Avenue, Seattle, WA 98101, USA.
Clin Immunol. 2008 Jul;128(1):23-30. doi: 10.1016/j.clim.2008.03.459. Epub 2008 May 2.
CD4+ T cells specific for the diabetes-associated autoantigen GAD65 were analyzed using peripheral blood samples after pancreas transplantation in subjects with T1D with clinical evidence of recurrent autoimmune diabetes. MHC class II tetramers facilitated the identification and cloning of antigen-specific autoreactive cells, which were found at several time points over a multiyear span, in spite of chronic immunosuppression of the subjects. Comparisons of TCR clonotypes by cDNA sequencing revealed that identical T cells were present in the circulation, separated by long time intervals, consistent with a persistent memory response associated with recurrent autoimmunity.
利用患有1型糖尿病且有复发性自身免疫性糖尿病临床证据的受试者胰腺移植后的外周血样本，对与糖尿病相关自身抗原GAD65特异性的CD4 + T细胞进行了分析。II类主要组织相容性复合体四聚体有助于鉴定和克隆抗原特异性自身反应性细胞，尽管这些受试者接受了长期免疫抑制，但在多年的多个时间点均发现了这些细胞。通过cDNA测序对TCR克隆型进行比较显示，相同的T细胞存在于循环中，间隔很长时间，这与复发性自身免疫相关的持续记忆反应一致。
