Mahlière Sophie, Perrin David, Peyronnet Julie, Boussouar Aurélien, Annat Guy, Viale Jean-Paul, Pequignot Jacqueline, Pequignot Jean-Marc, Dalmaz Yvette
Université de Lyon, Lyon, France.
Respir Physiol Neurobiol. 2008 Jun 30;162(1):32-40. doi: 10.1016/j.resp.2008.03.009. Epub 2008 Mar 28.
While perinatal nicotine effects on ventilation have been widely investigated, the prenatal impact of nicotine treatment during gestation on both breathing and neural circuits involved in respiratory control remains unknown. We examined the effects of nicotine, from embryonic day 5 (E5) to E20, on baseline ventilation, the two hypoxic ventilatory response components and in vivo tyrosine hydroxylase (TH) activity in carotid bodies and brainstem areas, assessed at postnatal day 7 (P7), P11 and P21. In pups prenatally exposed to nicotine, baseline ventilation and hypoxic ventilatory response were increased at P7 (+48%) and P11 (+46%), with increased tidal volume (p<0.05). Hypoxia blunted frequency response at P7 and revealed unstable ventilation at P11. In carotid bodies, TH activity increased by 20% at P7 and decreased by 48% at P11 (p<0.05). In most brainstem areas it was reduced by 20-33% until P11. Changes were resolved by P21. Prenatal nicotine led to postnatal ventilatory sequelae, partly resulting from impaired maturation of peripheral chemoreceptors and brainstem integrative sites.
虽然围产期尼古丁对通气的影响已得到广泛研究，但孕期尼古丁治疗对呼吸及参与呼吸控制的神经回路的产前影响仍不清楚。我们研究了从胚胎第5天（E5）到E20给予尼古丁对出生后第7天（P7）、P11和P21的基线通气、两种低氧通气反应成分以及颈动脉体和脑干区域体内酪氨酸羟化酶（TH）活性的影响。在产前暴露于尼古丁的幼崽中，P7（增加48%）和P11（增加46%）时基线通气和低氧通气反应增加，潮气量增加（p<0.05）。低氧使P7时的频率反应减弱，并在P11时显示通气不稳定。在颈动脉体中，TH活性在P7时增加20%，在P11时降低48%（p<0.05）。在大多数脑干区域，直到P11时其活性降低20 - 33%。这些变化在P21时恢复。产前尼古丁导致出生后通气后遗症，部分原因是外周化学感受器和脑干整合部位成熟受损。
