Bolland Benjamin J R F, Kanczler Janos M, Ginty Patrick J, Howdle Steve M, Shakesheff Kevin M, Dunlop Douglas G, Oreffo Richard O C
Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, UK.
Biomaterials. 2008 Aug;29(22):3221-7. doi: 10.1016/j.biomaterials.2008.04.017. Epub 2008 May 5.
Concerns over disease transmission, high costs and limited supply have led to interest in synthetic grafts in the field of impaction bone grafting (IBG). Poly(DL-lactic acid) (PLA) grafts are attractive alternatives due to their biocompatibility, established safety and versatile manufacturing process. This study examined the potential of PLA scaffolds augmented with human bone marrow stromal cells (HBMSCs) in IBG. In vitro and in vivo studies were performed on impacted morsellised PLA seeded with HBMSC and compared to PLA alone. In vitro samples were incubated under osteogenic conditions and in vivo samples were implanted subcutaneously into severely compromised immunodeficient mice, for 4 weeks. Biochemical, histological, mechanical and 3D micro-computed tomography analyses were performed. HBMSC viability, biochemical activity and histological evidence of osteogenic cellular differentiation, post-impaction were observed in vitro and in vivo in PLA/HBMSC samples compared to impacted PLA alone. In vitro PLA/HBMSC samples demonstrated evidence of mechanical enhancement over PLA alone. In vivo studies showed a significant increase in new bone and blood vessel formation in the PLA/HBMSC constructs compared to PLA alone. With alternatives to allograft being sought, these studies have demonstrated PLA/HBMSC living composites, to be a potential prospect as a biological bone graft extender for future use in the field of IBG.
对疾病传播、高成本和供应有限的担忧引发了人们对嵌压植骨（IBG）领域中合成移植物的兴趣。聚（DL-乳酸）（PLA）移植物因其生物相容性、既定的安全性和多样的制造工艺而成为有吸引力的替代品。本研究考察了用人骨髓基质细胞（HBMSC）增强的PLA支架在IBG中的潜力。对接种了HBMSC的嵌压碎粒状PLA进行了体外和体内研究，并与单独的PLA进行比较。体外样本在成骨条件下孵育，体内样本皮下植入严重免疫受损的小鼠体内，为期4周。进行了生化、组织学、力学和三维微计算机断层扫描分析。与单独的嵌压PLA相比，在体外和体内的PLA/HBMSC样本中均观察到HBMSC活力、生化活性和成骨细胞分化的组织学证据。体外PLA/HBMSC样本显示出比单独的PLA有机械增强的证据。体内研究表明，与单独的PLA相比，PLA/HBMSC构建体中的新骨和血管形成显著增加。随着对同种异体移植物替代品的探索，这些研究表明PLA/HBMSC活性复合材料作为一种生物骨移植扩展材料在未来IBG领域具有潜在的应用前景。
