Kanczler Janos, Tare Rahul S, Tribe Howard C, Stumpf Patrick, Rawlings Andrew, Marshall Karen, Oreffo Richard O C
Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development & Health, Institute of Developmental Sciences, University of Southampton Medical School, Southampton, UK.
Methods Mol Biol. 2025;2885:67-85. doi: 10.1007/978-1-0716-4306-8_4.
In this chapter, we describe techniques for the isolation, enrichment, and characterization of skeletal stem cells from human bone marrow. The methods for enriching STRO-1 and STRO-4 positive cells using magnetic activated cell sorting are detailed, as are the techniques for establishing and characterising osteogenic, adipogenic, and chondrogenic cultures derived from these cells. Finally, we present methods for studying the ability of these enriched skeletal stem and progenitor populations to produce bone in vivo using diffusion chambers following subcutaneous implantation, over 4-6 weeks.
在本章中，我们描述了从人骨髓中分离、富集和鉴定骨骼干细胞的技术。详细介绍了使用磁珠分选法富集STRO-1和STRO-4阳性细胞的方法，以及建立和鉴定源自这些细胞的成骨、成脂和成软骨培养物的技术。最后，我们介绍了在皮下植入扩散室4至6周后，研究这些富集的骨骼干祖细胞群体在体内生成骨能力的方法。
