• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Pitx2在血管平滑肌细胞分化的早期阶段具有重要功能。

Pitx2 is functionally important in the early stages of vascular smooth muscle cell differentiation.

作者信息

Shang Yueting, Yoshida Tadashi, Amendt Brad A, Martin James F, Owens Gary K

机构信息

Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA.

出版信息

J Cell Biol. 2008 May 5;181(3):461-73. doi: 10.1083/jcb.200711145.

DOI:10.1083/jcb.200711145
PMID:18458156
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2364692/
Abstract

Mechanisms that control vascular smooth muscle cell (SMC) differentiation are poorly understood. We identify Pitx2 as a previously unknown homeodomain transcription factor that is rapidly induced in an in vitro model of SMC differentiation from multipotent stem cells. Pitx2 induces expression of multiple SMC differentiation marker genes by binding to a TAATC(C/T) cis-element, by interacting with serum response factor, and by increasing histone acetylation levels within the promoters of SMC differentiation marker genes. Suppression of Pitx2 reduces expression of SMC differentiation marker genes in the early stages of SMC differentiation in vitro, whereas Prx1, another homeodomain protein, regulates SMC differentiation marker genes in fully differentiated SMCs. Pitx2, but not Prx1, knockout mouse embryos exhibit impaired induction of SMC differentiation markers in the dorsal aorta and branchial arch arteries. Our results demonstrate that Pitx2 functions to regulate the early stages of SMC differentiation.

摘要

控制血管平滑肌细胞（SMC）分化的机制目前仍知之甚少。我们发现Pitx2是一种此前未知的含同源结构域的转录因子，在多能干细胞向SMC分化的体外模型中它能被快速诱导表达。Pitx2通过与TAATC（C/T）顺式元件结合、与血清反应因子相互作用以及提高SMC分化标记基因启动子区域的组蛋白乙酰化水平，来诱导多个SMC分化标记基因的表达。在体外SMC分化的早期阶段，抑制Pitx2会降低SMC分化标记基因的表达，而另一种含同源结构域的蛋白Prx1则在完全分化的SMC中调节SMC分化标记基因。Pitx2基因敲除小鼠胚胎（而非Prx1基因敲除小鼠胚胎）在背主动脉和鳃弓动脉中表现出SMC分化标记诱导受损。我们的结果表明，Pitx2在调节SMC分化的早期阶段发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/f002b1ea108c/jcb1810461f08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/f0384752db06/jcb1810461f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/fad4008846fb/jcb1810461f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/3bdc802cff67/jcb1810461f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/c6a4ed293f97/jcb1810461f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/718cd3e8f0cd/jcb1810461f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/29bc752d8b06/jcb1810461f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/8f1ee8d672c6/jcb1810461f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/f002b1ea108c/jcb1810461f08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/f0384752db06/jcb1810461f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/fad4008846fb/jcb1810461f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/3bdc802cff67/jcb1810461f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/c6a4ed293f97/jcb1810461f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/718cd3e8f0cd/jcb1810461f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/29bc752d8b06/jcb1810461f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/8f1ee8d672c6/jcb1810461f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9cb1/2364692/f002b1ea108c/jcb1810461f08.jpg

相似文献

1
Pitx2 is functionally important in the early stages of vascular smooth muscle cell differentiation.Pitx2在血管平滑肌细胞分化的早期阶段具有重要功能。
J Cell Biol. 2008 May 5;181(3):461-73. doi: 10.1083/jcb.200711145.
2
Kruppel-like factor 4, Elk-1, and histone deacetylases cooperatively suppress smooth muscle cell differentiation markers in response to oxidized phospholipids.Kruppel样因子4、Elk-1和组蛋白去乙酰化酶协同抑制平滑肌细胞分化标志物以响应氧化磷脂。
Am J Physiol Cell Physiol. 2008 Nov;295(5):C1175-82. doi: 10.1152/ajpcell.00288.2008. Epub 2008 Sep 3.
3
PIAS1 activates the expression of smooth muscle cell differentiation marker genes by interacting with serum response factor and class I basic helix-loop-helix proteins.PIAS1 通过与血清反应因子及 I 类碱性螺旋-环-螺旋蛋白相互作用，激活平滑肌细胞分化标志物基因的表达。
Mol Cell Biol. 2005 Sep;25(18):8009-23. doi: 10.1128/MCB.25.18.8009-8023.2005.
4
Platelet-derived growth factor-BB represses smooth muscle cell marker genes via changes in binding of MKL factors and histone deacetylases to their promoters.血小板衍生生长因子-BB通过改变MKL因子和组蛋白去乙酰化酶与启动子的结合来抑制平滑肌细胞标记基因。
Am J Physiol Cell Physiol. 2007 Feb;292(2):C886-95. doi: 10.1152/ajpcell.00449.2006. Epub 2006 Sep 20.
5
G6PD activity contributes to the regulation of histone acetylation and gene expression in smooth muscle cells and to the pathogenesis of vascular diseases.葡萄糖-6-磷酸脱氢酶（G6PD）活性有助于调节平滑肌细胞中的组蛋白乙酰化和基因表达，并参与血管疾病的发病机制。
Am J Physiol Heart Circ Physiol. 2021 Mar 1;320(3):H999-H1016. doi: 10.1152/ajpheart.00488.2020. Epub 2021 Jan 8.
6
Recruitment of serum response factor and hyperacetylation of histones at smooth muscle-specific regulatory regions during differentiation of a novel P19-derived in vitro smooth muscle differentiation system.在一种新型的源自P19的体外平滑肌分化系统分化过程中，血清反应因子的募集以及平滑肌特异性调控区域组蛋白的高度乙酰化。
Circ Res. 2001 Jun 8;88(11):1127-34. doi: 10.1161/hh1101.091339.
7
Brain cytoplasmic RNA 1 suppresses smooth muscle differentiation and vascular development in mice.脑细胞质 RNA1 抑制小鼠平滑肌分化和血管发育。
J Biol Chem. 2018 Apr 13;293(15):5668-5678. doi: 10.1074/jbc.RA117.001578. Epub 2018 Feb 21.
8
Sp1-dependent activation of HDAC7 is required for platelet-derived growth factor-BB-induced smooth muscle cell differentiation from stem cells.Sp1 依赖性激活 HDAC7 是血小板衍生生长因子-BB 诱导干细胞向平滑肌细胞分化所必需的。
J Biol Chem. 2010 Dec 3;285(49):38463-72. doi: 10.1074/jbc.M110.153999. Epub 2010 Oct 1.
9
WD repeat-containing protein 5, a ubiquitously expressed histone methyltransferase adaptor protein, regulates smooth muscle cell-selective gene activation through interaction with pituitary homeobox 2.WD 重复蛋白 5 是一种广泛表达的组蛋白甲基转移酶衔接蛋白，通过与垂体同源盒 2 相互作用调节平滑肌细胞选择性基因激活。
J Biol Chem. 2011 Jun 17;286(24):21853-64. doi: 10.1074/jbc.M111.233098. Epub 2011 Apr 28.
10
Forced expression of myocardin is not sufficient for induction of smooth muscle differentiation in multipotential embryonic cells.心肌素的强制表达不足以诱导多能胚胎细胞向平滑肌分化。
Arterioscler Thromb Vasc Biol. 2004 Sep;24(9):1596-601. doi: 10.1161/01.ATV.0000137190.63214.c5. Epub 2004 Jul 1.

引用本文的文献

1
SWI/SNF Complex in Vascular Smooth Muscle Cells and Its Implications in Cardiovascular Pathologies.SWI/SNF 复合物在血管平滑肌细胞中的作用及其在心血管疾病中的意义。
Cells. 2024 Jan 16;13(2):168. doi: 10.3390/cells13020168.
2
Nuclear Control of Vascular Smooth Muscle Cell Plasticity during Vascular Remodeling.核对于血管重塑过程中血管平滑肌细胞可塑性的调控。
Am J Pathol. 2024 Apr;194(4):525-538. doi: 10.1016/j.ajpath.2023.09.013. Epub 2023 Oct 10.
3
Human Induced Pluripotent Stem Cell-Derived Vascular Cells: Recent Progress and Future Directions.

本文引用的文献

1
MCAT elements and the TEF-1 family of transcription factors in muscle development and disease.肌肉发育与疾病中的MCAT元件及转录因子TEF-1家族
Arterioscler Thromb Vasc Biol. 2008 Jan;28(1):8-17. doi: 10.1161/ATVBAHA.107.155788. Epub 2007 Oct 25.
2
Myocardin is a bifunctional switch for smooth versus skeletal muscle differentiation.心肌素是平滑肌与骨骼肌分化的双功能开关。
Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16570-5. doi: 10.1073/pnas.0708253104. Epub 2007 Oct 10.
3
Smooth muscle cells and myofibroblasts use distinct transcriptional mechanisms for smooth muscle alpha-actin expression.
人诱导多能干细胞衍生的血管细胞：最新进展与未来方向
J Cardiovasc Dev Dis. 2021 Nov 4;8(11):148. doi: 10.3390/jcdd8110148.
4
PRRX1 deficiency induces mesenchymal-epithelial transition through PITX2/miR-200-dependent SLUG/CTNNB1 regulation in hepatocellular carcinoma.PRRX1 缺失通过 PITX2/miR-200 依赖的 SLUG/CTNNB1 调控诱导肝癌中的间质-上皮转化。
Cancer Sci. 2021 Jun;112(6):2158-2172. doi: 10.1111/cas.14853. Epub 2021 Apr 8.
5
Circulating anti-geronic factors from heterochonic parabionts promote vascular rejuvenation in aged mice: transcriptional footprint of mitochondrial protection, attenuation of oxidative stress, and rescue of endothelial function by young blood.来自异时共生异体的循环抗衰老因子促进老年小鼠的血管年轻化：通过年轻血液实现线粒体保护、氧化应激减弱和内皮功能恢复的转录足迹。
Geroscience. 2020 Apr;42(2):727-748. doi: 10.1007/s11357-020-00180-6. Epub 2020 Mar 15.
6
Transcription factor TEAD1 is essential for vascular development by promoting vascular smooth muscle differentiation.转录因子 TEAD1 通过促进血管平滑肌分化对于血管发育是必需的。
Cell Death Differ. 2019 Dec;26(12):2790-2806. doi: 10.1038/s41418-019-0335-4. Epub 2019 Apr 25.
7
gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting .gga-微核糖核酸-133a-3p通过靶向……调控成肌细胞增殖与分化
Front Genet. 2018 Dec 4;9:577. doi: 10.3389/fgene.2018.00577. eCollection 2018.
8
Lineage dynamics of murine pancreatic development at single-cell resolution.单细胞分辨率解析的小鼠胰腺发育的谱系动力学。
Nat Commun. 2018 Sep 25;9(1):3922. doi: 10.1038/s41467-018-06176-3.
9
Smooth Muscle-Selective Nuclear Factor-κB Inhibition Reduces Phosphate-Induced Arterial Medial Calcification in Mice With Chronic Kidney Disease.平滑肌选择性核因子-κB 抑制可减少慢性肾脏病小鼠磷酸盐诱导的动脉中层钙化。
J Am Heart Assoc. 2017 Nov 16;6(11):e007248. doi: 10.1161/JAHA.117.007248.
10
Gene expression profiles and signaling mechanisms in α-adrenoceptor-evoked proliferation of vascular smooth muscle cells.α-肾上腺素能受体诱发血管平滑肌细胞增殖中的基因表达谱及信号传导机制
BMC Syst Biol. 2017 Jun 28;11(1):65. doi: 10.1186/s12918-017-0439-8.
平滑肌细胞和成肌纤维细胞利用不同的转录机制来表达平滑肌α-肌动蛋白。
Circ Res. 2007 Oct 26;101(9):883-92. doi: 10.1161/CIRCRESAHA.107.154831. Epub 2007 Sep 6.
4
Platelet-derived growth factor-BB represses smooth muscle cell marker genes via changes in binding of MKL factors and histone deacetylases to their promoters.血小板衍生生长因子-BB通过改变MKL因子和组蛋白去乙酰化酶与启动子的结合来抑制平滑肌细胞标记基因。
Am J Physiol Cell Physiol. 2007 Feb;292(2):C886-95. doi: 10.1152/ajpcell.00449.2006. Epub 2006 Sep 20.
5
Pitx2 regulates cardiac left-right asymmetry by patterning second cardiac lineage-derived myocardium.Pitx2 通过调控第二心脏谱系衍生的心肌细胞模式来调节心脏左右不对称性。
Dev Biol. 2006 Aug 15;296(2):437-49. doi: 10.1016/j.ydbio.2006.06.009. Epub 2006 Jun 14.
6
Assessment of contractility of purified smooth muscle cells derived from embryonic stem cells.对源自胚胎干细胞的纯化平滑肌细胞收缩性的评估。
Stem Cells. 2006 Jul;24(7):1678-88. doi: 10.1634/stemcells.2006-0002. Epub 2006 Apr 6.
7
Stem cells and their derivatives can bypass the requirement of myocardin for smooth muscle gene expression.干细胞及其衍生物可以绕过对心肌蛋白的需求来实现平滑肌基因表达。
Dev Biol. 2005 Dec 15;288(2):502-13. doi: 10.1016/j.ydbio.2005.10.014. Epub 2005 Nov 23.
8
Requirement of myocardin-related transcription factor-B for remodeling of branchial arch arteries and smooth muscle differentiation.心肌相关转录因子-B对鳃弓动脉重塑和平滑肌分化的需求。
Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15122-7. doi: 10.1073/pnas.0507346102. Epub 2005 Oct 4.
9
Expression of the homeobox gene Pitx2 in neural crest is required for optic stalk and ocular anterior segment development.同源框基因Pitx2在神经嵴中的表达对视神经柄和眼前节发育是必需的。
Hum Mol Genet. 2005 Nov 15;14(22):3347-59. doi: 10.1093/hmg/ddi365. Epub 2005 Oct 3.
10
Myocardin-related transcription factor B is required in cardiac neural crest for smooth muscle differentiation and cardiovascular development.心肌相关转录因子B是心脏神经嵴平滑肌分化和心血管发育所必需的。
Proc Natl Acad Sci U S A. 2005 Jun 21;102(25):8916-21. doi: 10.1073/pnas.0503741102. Epub 2005 Jun 10.