Tang Yin, Cao Chuanbao, Ma Xilan, Chen Chen, Zhu Hesun
Research Center of Material Science, Beijing Institute of Technology, Bejing 100081, People's Republic of China.
Biomed Mater. 2006 Dec;1(4):242-6. doi: 10.1088/1748-6041/1/4/010. Epub 2006 Dec 4.
Blended films were prepared from a silk fibroin (SF) solution by adding a small amount of type I collagen (<5%). The mechanical properties of the wet films modified by collagen were improved obviously. The elongation at break reached 42%, and the smaller contact angles revealed that modified films had better hydrophilicity. 1% heparin was also added to modify the SF films to further improve the in vitro antithrombogenecity. The internal structure of the modified SF films was investigated with scanning electron microscopy, x-ray diffraction and Fourier transform infrared attenuated total reflection spectroscopy. The result indicates that the addition of a small amount of collagen and heparin did not change their conformation.
通过添加少量I型胶原蛋白（<5%），由丝素蛋白（SF）溶液制备混合膜。经胶原蛋白改性的湿膜的机械性能明显提高。断裂伸长率达到42%，较小的接触角表明改性膜具有更好的亲水性。还添加1%的肝素对SF膜进行改性，以进一步提高其体外抗血栓形成能力。用扫描电子显微镜、X射线衍射和傅里叶变换红外衰减全反射光谱对改性SF膜的内部结构进行了研究。结果表明，添加少量胶原蛋白和肝素并没有改变它们的构象。
