Mandal Biman B, Mann Jasdeep K, Kundu S C
Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, West Bengal, India.
Eur J Pharm Sci. 2009 May 12;37(2):160-71. doi: 10.1016/j.ejps.2009.02.005. Epub 2009 Feb 21.
Multilayer films based on silk fibroin protein and gelatin was fabricated in aqueous solution for controlled drug release. The gradual build up of layer was investigated by UV-vis spectroscopy and was further analyzed through attenuated total reflectance-Fourier transform infrared spectroscopy. Scanning electron microscopy confirmed the presence of distinct layers within the multilayer system. Intervening dehydrating step by methanol treatment was used to control the structure and stability of the self-assembled silk fibroin/gelatin multilayer films. The films were tested for in vitro release using three different molecular weight model compounds namely trypan blue (961 Da), FITC-inulin (3.9 kDa) and FITC-BSA (66 kDa). The release profile of compounds revealed dependence on multilayer film degradation for sustained release. The release kinetics was further evaluated as a function of gelatin and buildup of layers suggesting their possible role in restricting initial burst leading to sustained compound release. MTT and confocal microscopy were used to assess cellular viability and biocompatibility of fabricated films using fibroblast cells. The results highlight the versatile and tunable properties of fibroin/gelatin multilayer films making them exciting candidates for the controlled release of a wide spectrum of bioactive molecules.
基于丝素蛋白和明胶的多层膜在水溶液中制备用于药物控释。通过紫外可见光谱研究了层的逐步形成，并通过衰减全反射傅里叶变换红外光谱进一步分析。扫描电子显微镜证实了多层体系中存在不同的层。采用甲醇处理的中间脱水步骤来控制自组装丝素蛋白/明胶多层膜的结构和稳定性。使用三种不同分子量的模型化合物，即台盼蓝（961 Da）、异硫氰酸荧光素标记的菊粉（3.9 kDa）和异硫氰酸荧光素标记的牛血清白蛋白（66 kDa）对薄膜进行体外释放测试。化合物的释放曲线表明其依赖于多层膜降解以实现持续释放。进一步评估了释放动力学作为明胶和层数的函数，表明它们在限制初始突释从而导致化合物持续释放方面可能发挥的作用。使用MTT和共聚焦显微镜评估了使用成纤维细胞制备的薄膜的细胞活力和生物相容性。结果突出了丝素蛋白/明胶多层膜的多功能和可调节特性，使其成为广泛生物活性分子控释的令人兴奋的候选材料。
