通过等离子体技术接枝胶原蛋白来改善聚（D，L-丙交酯）薄膜的细胞亲和力。

Improving the cell affinity of a poly(D,L-lactide) film modified by grafting collagen via a plasma technique.

作者信息

Zhao Jian-Hao, Wang Jue, Tu Mei, Luo Bing-Hong, Zhou Chang-Ren

机构信息

Department of Material Science and Engineering, College of Science and Engineering, Jinan University, 510632, Guangzhou, People's Republic of China.

出版信息

Biomed Mater. 2006 Dec;1(4):247-52. doi: 10.1088/1748-6041/1/4/011. Epub 2006 Dec 4.

DOI:10.1088/1748-6041/1/4/011

PMID:18458413

Abstract

Poly(D,L-lactide) films were surface-modified by grafting collagen via NH(3) plasma to improve cell affinity. The modified films were characterized by IR analysis, contact angle measurement, SEM analysis and collagen quantity determination. It was demonstrated that -NH(2) and collagen were incorporated into the surface of PDLLA films. The hydrophilicity of the PDLLA film increased after NH(3) plasma treatment, but decreased with further collagen modification. More collagen was incorporated into the PDLLA films by a grating method as compared to that with an anchorage treatment. L929 fibroblast cells were used to evaluate the cell affinity of the modified films and control. It was shown that PDLLA films surface-modified by grafting collagen via NH(3) plasma more efficiently enhanced the cells attachment and proliferation than those films modified by collagen anchorage or only NH(3) plasma treatment.

摘要

通过NH₃等离子体接枝胶原蛋白对聚（D，L-丙交酯）薄膜进行表面改性，以提高细胞亲和力。通过红外分析、接触角测量、扫描电子显微镜分析和胶原蛋白含量测定对改性薄膜进行表征。结果表明，-NH₂和胶原蛋白被引入到聚-D，L-丙交酯（PDLLA）薄膜表面。NH₃等离子体处理后，PDLLA薄膜的亲水性增加，但随着进一步的胶原蛋白改性而降低。与锚定处理相比，通过光栅法可使更多的胶原蛋白掺入PDLLA薄膜中。使用L929成纤维细胞评估改性薄膜和对照的细胞亲和力。结果表明，通过NH₃等离子体接枝胶原蛋白表面改性的PDLLA薄膜比通过胶原蛋白锚定或仅NH₃等离子体处理改性的薄膜更有效地促进细胞附着和增殖。