Wang Jing, Liu Changsheng, Wei Jie, Chi Ping, Lu Xin, Yin Min
Engineering Research Center for Biomedical Materials of Ministry of Education, Institute of Biomedical Materials, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
Biomed Mater. 2007 Mar;2(1):32-8. doi: 10.1088/1748-6041/2/1/006. Epub 2007 Jan 19.
Adhesive polypeptide containing the key component of mussel adhesive protein was synthesized by ring-opening polymerization, and a hybrid material of the adhesive polypeptide and chitosan was prepared through the solution method. Some strong hydrogen bond interaction existed, but without the chemical bond between chitosan and polypeptide molecular in the composites, which was demonstrated by IR and XRD. Tensile strength and elongation-at-break of the composite increased with the increase of the polypeptide content. However, the mechanical properties decreased when the content of polypeptide was more than 2% in the composite; all in all, the mechanical properties of the composite were better than the pure chitosan. Furthermore, the introduction of polypeptide was beneficial in improving the hydrophilicity and cell affinity of the composite. The results indicated that the novel chitosan/polypeptide composite has excellent biocompatibility, which could be a scaffold material for cell culture in tissue engineering.
通过开环聚合合成了含有贻贝粘附蛋白关键成分的粘附多肽，并采用溶液法制备了粘附多肽与壳聚糖的杂化材料。红外光谱（IR）和X射线衍射（XRD）表明，复合材料中存在一些强氢键相互作用，但壳聚糖与多肽分子之间不存在化学键。复合材料的拉伸强度和断裂伸长率随多肽含量的增加而提高。然而，当复合材料中多肽含量超过2%时，力学性能下降；总体而言，复合材料的力学性能优于纯壳聚糖。此外，多肽的引入有利于提高复合材料的亲水性和细胞亲和力。结果表明，新型壳聚糖/多肽复合材料具有优异的生物相容性，可作为组织工程中细胞培养的支架材料。
