Al-Omari S
Department of Physics, The Hashemite University, Zarqa 13115, Jordan.
Biomed Mater. 2007 Jun;2(2):107-15. doi: 10.1088/1748-6041/2/2/008. Epub 2007 Mar 14.
Some of the photophysical properties (stationary absorbance and fluorescence, fluorescence decay times and singlet oxygen quantum yields) of chlorins substituted with methoxy groups, hydroxyl groups and hydrocarbonic chains were studied in ethanol and dipalmitoyl-phosphatidylcholine (DPPC) liposomes using steady-state and time-resolved fluorescence spectroscopies. The photophysical behaviors of the chlorins in liposomes like cellular membrane were compared with those obtained from chlorin-liposome systems delivered to Jurkat cells in order to select potent photosensitizers for the photodynamic treatment of cancer. The localization of the studied chlorins inside liposomes was found to depend strongly on the substituents of chlorins. Absorption spectra of chlorins embedded in DPPC-liposomes have been recorded in the temperature range of 20-70 degrees C. It is demonstrated that the location of the chlorin molecules depends on the phase state of the phospholipids. These observations are confirmed by the fluorescence lifetimes, singlet oxygen lifetimes and singlet oxygen quantum yields results.
利用稳态和时间分辨荧光光谱，研究了在乙醇和二棕榈酰磷脂酰胆碱（DPPC）脂质体中，被甲氧基、羟基和烃链取代的二氢卟吩的一些光物理性质（稳态吸收和荧光、荧光衰减时间和单线态氧量子产率）。将二氢卟吩在类似细胞膜的脂质体中的光物理行为与从递送至Jurkat细胞的二氢卟吩-脂质体系统中获得的行为进行比较，以选择用于癌症光动力治疗的有效光敏剂。发现所研究的二氢卟吩在脂质体内的定位强烈依赖于二氢卟吩的取代基。已记录了嵌入DPPC脂质体中的二氢卟吩在20至70摄氏度温度范围内的吸收光谱。结果表明，二氢卟吩分子的位置取决于磷脂的相态。这些观察结果通过荧光寿命、单线态氧寿命和单线态氧量子产率结果得到证实。
