Ito Atsuo, Sogo Yu, Ebihara Yuko, Onoguchi Masahiro, Oyane Ayako, Ichinose Noboru
National Institute of Advanced Industrial Science and Technology (AIST), Institute for Human Science and Biomedical Engineering, Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
Biomed Mater. 2007 Sep;2(3):S181-5. doi: 10.1088/1748-6041/2/3/S17. Epub 2007 Jul 30.
An ascorbate-apatite composite layer was successfully formed on NaOH- and heat-treated titanium by coprecipitating L-ascorbic acid phosphate and low-crystalline apatite in a supersaturated calcium phosphate solution at 37 degrees C for 48 h. The supersaturated calcium phosphate solutions used have chemical compositions attainable by mixing infusion fluids officially approved for clinical use. The amount of immobilized L-ascorbic acid phosphate ranged from 1.0 to 2.3 microg mm(-2), which is most likely to be sufficient for the in vitro osteogenic differentiation of mesenchymal stem cells on titanium. Since ascorbate is important for the collagen synthesis and subsequent osteogenesis of mesenchymal stem cells, titanium coated with the ascorbate-apatite composite layer would be useful as a scaffold in bone tissue engineering and as a bone substitute.
通过在37℃下于过饱和磷酸钙溶液中使L-抗坏血酸磷酸酯和低结晶度磷灰石共沉淀48小时，在经氢氧化钠和热处理的钛表面成功形成了抗坏血酸-磷灰石复合层。所使用的过饱和磷酸钙溶液的化学组成可通过混合临床使用的官方批准输液获得。固定化L-抗坏血酸磷酸酯的量在1.0至2.3微克/平方毫米范围内，这很可能足以促进间充质干细胞在钛表面的体外成骨分化。由于抗坏血酸对间充质干细胞的胶原蛋白合成及随后的骨生成很重要，涂覆有抗坏血酸-磷灰石复合层的钛将可作为骨组织工程中的支架和骨替代物。
