Vaughan S P, Grisoni M, Kumagai M H, Kuehnle A R
Department of Tropical Plant and Soil Sciences, University of Hawaii, 3190 Maile Way, Honolulu, HI 98622, USA.
Arch Virol. 2008;153(6):1185-9. doi: 10.1007/s00705-008-0102-1. Epub 2008 May 6.
We report here the isolation and characterization of three distinct isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid in Hawaii. Isolates 1 and 2 were phylogenetically distinct from previously reported CymMV isolates. However, isolate 3 was highly similar to previously reported CymMV sequences and could be localised to CymMV subgroup A. Isolate 2 localised to CymMV subgroup B. Thus, we report here the first full-length CymMV subgroup B isolate. Isolate 1 represents a recombination event between isolates 2 and 3. Infectivity assays revealed that all three isolates are functional and individually infectious in both Dendrobium and indicator species.
我们在此报告了在夏威夷共感染石斛兰的三种不同的建兰花叶病毒（CymMV）分离株的分离与特性鉴定。分离株1和2在系统发育上与先前报道的CymMV分离株不同。然而，分离株3与先前报道的CymMV序列高度相似，可归为CymMV A亚组。分离株2归为CymMV B亚组。因此，我们在此报告了首个全长的CymMV B亚组分离株。分离株1代表了分离株2和3之间的重组事件。感染性测定表明，所有三种分离株均具有功能，且在石斛兰和指示物种中均具有单独的感染性。
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