Iwao Keiichiro, Inatani Masaru, Okinami Satoshi, Tanihara Hidenobu
Department of Ophthalmology and Visual Science, Kumamoto University Graduate School of Medical Sciences, 1-1-1, Honjo, 860-8556, Kumamoto, Japan.
Graefes Arch Clin Exp Ophthalmol. 2008 Aug;246(8):1117-22. doi: 10.1007/s00417-008-0845-0. Epub 2008 May 6.
To map neural crest cell fate during eye development.
Neural crest cells were tracked in developing mouse eyes using a transgene expressing Cre recombinase controlled by the Protein 0 promoter and a Rosa26 Cre-responsive reporter gene that produced beta-galactosidase after Cre-mediated recombination.
beta-galactosidase-positive cells were detected in the periocular segment on embryonic day (E) 9.5. Several neural crest cell-derived tissues including corneal stroma, corneal endothelium, iridocorneal angle, ciliary body, primary vitreous and eyelid were strongly stained on E13.5-E18.5. The staining decreased in the corneal stroma after birth, but persisted in the presumptive iridocorneal angle.
Protein 0-Cre transgenic mice offer a conditional knock-out strategy to investigate anterior eye segment differentiation.
绘制眼部发育过程中神经嵴细胞的命运图谱。
利用由蛋白0启动子控制表达Cre重组酶的转基因和Rosa26 Cre反应性报告基因，在发育中的小鼠眼睛中追踪神经嵴细胞，该报告基因在Cre介导的重组后产生β-半乳糖苷酶。
在胚胎第（E）9.5天的眼周段检测到β-半乳糖苷酶阳性细胞。在E13.5 - E18.5时，包括角膜基质、角膜内皮、虹膜角膜角、睫状体、原始玻璃体和眼睑在内的几种神经嵴细胞衍生组织被强烈染色。出生后角膜基质中的染色减少，但在假定的虹膜角膜角中持续存在。
蛋白0 - Cre转基因小鼠提供了一种条件性敲除策略来研究眼前节分化。
