利用蛋白质0启动子驱动的转基因技术对眼发育过程中神经嵴细胞进行命运图谱分析。
Fate mapping of neural crest cells during eye development using a protein 0 promoter-driven transgenic technique.
作者信息
Iwao Keiichiro, Inatani Masaru, Okinami Satoshi, Tanihara Hidenobu
机构信息
Department of Ophthalmology and Visual Science, Kumamoto University Graduate School of Medical Sciences, 1-1-1, Honjo, 860-8556, Kumamoto, Japan.
出版信息
Graefes Arch Clin Exp Ophthalmol. 2008 Aug;246(8):1117-22. doi: 10.1007/s00417-008-0845-0. Epub 2008 May 6.
PURPOSE
To map neural crest cell fate during eye development.
METHODS
Neural crest cells were tracked in developing mouse eyes using a transgene expressing Cre recombinase controlled by the Protein 0 promoter and a Rosa26 Cre-responsive reporter gene that produced beta-galactosidase after Cre-mediated recombination.
RESULTS
beta-galactosidase-positive cells were detected in the periocular segment on embryonic day (E) 9.5. Several neural crest cell-derived tissues including corneal stroma, corneal endothelium, iridocorneal angle, ciliary body, primary vitreous and eyelid were strongly stained on E13.5-E18.5. The staining decreased in the corneal stroma after birth, but persisted in the presumptive iridocorneal angle.
CONCLUSIONS
Protein 0-Cre transgenic mice offer a conditional knock-out strategy to investigate anterior eye segment differentiation.
目的
绘制眼部发育过程中神经嵴细胞的命运图谱。
方法
利用由蛋白0启动子控制表达Cre重组酶的转基因和Rosa26 Cre反应性报告基因，在发育中的小鼠眼睛中追踪神经嵴细胞，该报告基因在Cre介导的重组后产生β-半乳糖苷酶。
结果
在胚胎第（E）9.5天的眼周段检测到β-半乳糖苷酶阳性细胞。在E13.5 - E18.5时，包括角膜基质、角膜内皮、虹膜角膜角、睫状体、原始玻璃体和眼睑在内的几种神经嵴细胞衍生组织被强烈染色。出生后角膜基质中的染色减少，但在假定的虹膜角膜角中持续存在。
结论
蛋白0 - Cre转基因小鼠提供了一种条件性敲除策略来研究眼前节分化。
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