Wintzer H O, Zipfel I, Schulte-Mönting J, Hellerich U, von Kleist S
Institute of Immunobiology, University of Freiburg, Federal Republic of Germany.
Cancer. 1991 Jan 15;67(2):421-8. doi: 10.1002/1097-0142(19910115)67:2<421::aid-cncr2820670217>3.0.co;2-q.
Ki-67 labeling was quantified in 37 nonmalignant breast tissues and in 63 breast carcinomas by counting ten random high-power fields each in three section planes (RC) or by evaluation of the area with the highest labeling density (HDC). Both procedures proved to be highly correlated (rs = 0.94). Ki-67-positive fractions of the nonmalignant tissues (mean, 2.1% for RC and 4.1% for HDC) were significantly lower as compared with the carcinomas (mean, 14.5% for RC and 17.5% for HDC). In carcinomas the Ki-67 labeling was significantly associated with pT stage, axillary lymph node status, and tumor grading and inversely related to progesterone receptor status. Using the medians of both counting methods (12% for RC and 17% for HDC) as cutoff points, significantly different curves for overall and disease-free survival (median follow-up, 37 months) were obtained. However, Cox multivariate analysis failed to demonstrate an independent effect of Ki-67 labeling. In contrast, Ki-67 reactivity seems to be of independent prognostic value if a higher cutoff level was selected.
通过在三个切片平面（RC）中各计数十个随机高倍视野，或通过评估标记密度最高的区域（HDC），对37例非恶性乳腺组织和63例乳腺癌组织中的Ki-67标记进行定量分析。两种方法的结果高度相关（rs = 0.94）。与癌组织相比，非恶性组织的Ki-67阳性率（RC法平均为2.1%，HDC法平均为4.1%）显著更低（癌组织RC法平均为14.5%，HDC法平均为17.5%）。在癌组织中，Ki-67标记与pT分期、腋窝淋巴结状态及肿瘤分级显著相关，与孕激素受体状态呈负相关。以两种计数方法的中位数（RC法为12%，HDC法为17%）作为截断点，得出了总生存期和无病生存期（中位随访时间37个月）的显著不同曲线。然而，Cox多因素分析未能证明Ki-67标记具有独立影响。相反，如果选择更高的截断水平，Ki-67反应性似乎具有独立的预后价值。
