Ran M, Katz B, Kimchi N, Halachmi E, Teillaud J L, Even J, Berko-Flint Y, Atlas E, Fridman W H, Witz I P
Department of Microbiology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
Cancer Res. 1991 Jan 15;51(2):612-8.
BALB/c 3T3 cells transformed in vitro with polyoma virus were cloned and passaged once in syngeneic mice. Resulting tumors from each clone were explanted and recultured. Expression of receptor for Fc of IgG (Fc gamma RII) in the original in vitro maintained clones and in cells derived from tumors elicited by the respective cells was measured at the protein level as well as at the mRNA level. Clones were assayed in pairs. The ancestor in vitro maintained clones [designated cultured cells (C)] were compared with cells derived from the same clones after a single passage in vivo followed by explantation and reculturing [designated cultured-tumor-cultured cells (CTC)]. C cells of any of the tested clones did not express Fc gamma RII. On the other hand, certain CTC cells were positive. The Fc gamma RII-positive cells were derived from tumors appearing after a long precancer latency period (greater than 140 days). CTC cells derived from tumors that appeared after shorter latency periods (less than 80 days) were Fc gamma RII negative. These results were obtained both by using radioimmunoassay and monoclonal antibodies against mouse Fc gamma RII as well as by Northern blot analysis using the Fc gamma RII complementary DNA probe. The involvement of macrophages as the Fc gamma RII-expressing cells in CTC cells was excluded. Fc gamma RII expression was down-regulated in CTC cells as a function of time following their explantation into culture. Fc gamma RII expression could be up-regulated in these cells and induced on C cells by maintaining the cultured cells in the presence of normal mouse serum or recombinant interferon. We also tested the expression of Fc gamma RII on CTC cells following their inoculation into syngeneic mice for a second time (CTCx2 cells). The results showed a positive correlation between Fc gamma RII expression in the inoculated ancestor CTC cells and on the CTCx2 cell progeny.
用多瘤病毒体外转化的BALB/c 3T3细胞被克隆，并在同基因小鼠中传代一次。每个克隆产生的肿瘤被取出并重新培养。在蛋白质水平和mRNA水平上测量原始体外培养克隆以及由相应细胞引发的肿瘤衍生细胞中IgG的Fc受体（FcγRII）的表达。成对检测克隆。将体外培养的原始克隆[称为培养细胞（C）]与在体内单次传代后取出并重新培养的同一克隆衍生的细胞[称为培养-肿瘤-培养细胞（CTC）]进行比较。任何测试克隆的C细胞均不表达FcγRII。另一方面，某些CTC细胞呈阳性。FcγRII阳性细胞来源于癌前潜伏期较长（超过140天）后出现的肿瘤。潜伏期较短（少于80天）后出现的肿瘤衍生的CTC细胞FcγRII呈阴性。这些结果通过使用放射免疫测定和抗小鼠FcγRII单克隆抗体以及使用FcγRII互补DNA探针的Northern印迹分析获得。排除了巨噬细胞作为CTC细胞中表达FcγRII的细胞的参与。FcγRII表达在CTC细胞重新接种到培养基后随时间下调。通过在正常小鼠血清或重组干扰素存在下培养细胞，可使这些细胞中FcγRII表达上调并在C细胞上诱导表达。我们还测试了CTC细胞再次接种到同基因小鼠（CTCx2细胞）后FcγRII的表达。结果显示接种的原始CTC细胞和CTCx2细胞后代中FcγRII表达之间存在正相关。
