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生物素化反义甲基膦酸酯寡脱氧核苷酸。剪接体组装的抑制及U1和U2小核核糖核蛋白的亲和选择。

Biotinylated antisense methylphosphonate oligodeoxynucleotides. Inhibition of spliceosome assembly and affinity selection of U1 and U2 small nuclear RNPs.

作者信息

Temsamani J, Agrawal S, Pederson T

机构信息

Cell Biology Group, The Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.

出版信息

J Biol Chem. 1991 Jan 5;266(1):468-72.

PMID:1845976
Abstract

Methylphosphonate (PC) backbone oligodeoxynucleotides complementary to the 5'-terminal nucleotides of U1 and U2 small nuclear (sn) RNAs do not elicit RNase H action under conditions in which natural (phosphodiester) oligodeoxynucleotides yield extensive RNase H cleavage. We show here that antisense PC oligonucleotides can mask sites in U1 and U2 snRNPs that are required for spliceosome formation. We further report that biotinylated derivatives of antisense PC oligos can be used for affinity selection of U1 and U2 snRNPs.

摘要

与U1和U2小核（sn）RNA的5'-末端核苷酸互补的甲基膦酸酯（PC）骨架寡脱氧核苷酸，在天然（磷酸二酯）寡脱氧核苷酸产生广泛的核糖核酸酶H切割的条件下，不会引发核糖核酸酶H的作用。我们在此表明，反义PC寡核苷酸可以掩盖剪接体形成所需的U1和U2 snRNP中的位点。我们还进一步报道，反义PC寡核苷酸的生物素化衍生物可用于U1和U2 snRNP的亲和选择。

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Biotinylated antisense methylphosphonate oligodeoxynucleotides. Inhibition of spliceosome assembly and affinity selection of U1 and U2 small nuclear RNPs.生物素化反义甲基膦酸酯寡脱氧核苷酸。剪接体组装的抑制及U1和U2小核核糖核蛋白的亲和选择。
J Biol Chem. 1991 Jan 5;266(1):468-72.
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引用本文的文献

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Antisense probes targeted to an internal domain in U2 snRNP specifically inhibit the second step of pre-mRNA splicing.
Nucleic Acids Res. 1992 Sep 11;20(17):4457-64. doi: 10.1093/nar/20.17.4457.