Shi M L, Li H Y, Schubert J, Zhou X P
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
Acta Virol. 2008;52(1):59-62.
Fourteen isolates of Turnip mosaic virus (TuMV) were obtained from the leaves of diseased cruciferous plants in China. According host tests, the isolates were classified into B-host and BR-host group. The nucleotide sequences of the coat protein (CP) and helper component proteinase (HC-Pro) genes of the isolates were determined. The CP genes consisted of 864 nucleotides encoding a polypeptide of 288 amino acids. The HC-Pro genes comprised 1374 nucleotides encoding a polypeptide of 458 amino acids. The genes CP and HC-Pro of the 14 isolates shared nucleotide sequence identities ranging from 89.2 to 99.5% and 79.1 to 99.9%, respectively. Amino acid sequence identities of CP and HC-Pro proteins ranged from 95.1 to 100% and 94.8 to 99.8%, respectively. Phylogenetic tree based on the CP gene indicated that 13 of the 14 TuMV isolates belonged to the world-B group, while the remaining isolate ZJ1 belonged to the basal-BR group. The phylogenetic tree based on the HC-Pro gene was similar to that of CP gene with the exception of the isolate JX that clustered with the Asian-BR group. Our results were consistent with the previous results demonstrating that a majority of the isolates collected from Brassica spp. belonged to the world-B group.
从中国患病十字花科植物叶片中获得了14株芜菁花叶病毒（TuMV）分离株。根据寄主测试，这些分离株被分为B寄主组和BR寄主组。测定了这些分离株的外壳蛋白（CP）基因和辅助成分蛋白酶（HC-Pro）基因的核苷酸序列。CP基因由864个核苷酸组成，编码一个含288个氨基酸的多肽。HC-Pro基因由1374个核苷酸组成，编码一个含458个氨基酸的多肽。14个分离株的CP基因和HC-Pro基因的核苷酸序列同一性分别为89.2%至99.5%和79.1%至99.9%。CP蛋白和HC-Pro蛋白的氨基酸序列同一性分别为95.1%至100%和94.8%至99.8%。基于CP基因构建的系统发育树表明，14个TuMV分离株中的13个属于世界B组，而其余的ZJ1分离株属于基部BR组。基于HC-Pro基因构建的系统发育树与CP基因的相似，但JX分离株与亚洲BR组聚类。我们的结果与之前的结果一致，表明从芸苔属植物中收集的大多数分离株属于世界B组。
