Sánchez F, Rodríguez-Mateos M, Touriño A, Fresno J, Gómez-Campo C, Jenner C E, Walsh J A, Ponz F
Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Madrid, Spain.
Arch Virol. 2007;152(6):1061-8. doi: 10.1007/s00705-007-0943-z. Epub 2007 Mar 9.
Turnip mosaic virus (TuMV) was found infecting cultivated brassicas and wild and cultivated ornamental Brassicaceae plants in different regions of Spain. Five new TuMV isolates, originating from different host plant species (Brassica cretica, Brassica juncea, Brassica napus, Eruca vesicaria subsp. sativa and Sisymbrium orientale), have been identified. The nucleotide sequences of the coat protein (CP) genes of the five isolates were determined. Phylogenetic analysis of the CP sequences showed that the five isolates grouped into two different clusters. The three isolates from the central region of Spain clustered with a previously reported Pisum sativum isolate from southeastern Spain, whereas the other two isolates from the eastern region clustered with two Italian and two Greek isolates. Both clusters were genetically distinct and belonged to the multi-lineage group OBR. The OBR group contains mainly TuMV isolates from hosts other than Brassica spp. and Raphanus sativus and mostly originating from Mediterranean countries. These new sequences provide further phylogenetic resolution of the OBR group. Although new TuMV isolates have been found in Spain, they were not associated with any serious disease outbreaks.
在西班牙不同地区发现芜菁花叶病毒（TuMV）感染栽培的芸苔属植物以及野生和栽培的十字花科观赏植物。已鉴定出五个新的TuMV分离株，它们源自不同的寄主植物物种（克里特岛芸苔、芥菜、甘蓝型油菜、芝麻菜和东方芝麻菜）。测定了这五个分离株的外壳蛋白（CP）基因的核苷酸序列。CP序列的系统发育分析表明，这五个分离株分为两个不同的簇。来自西班牙中部地区的三个分离株与先前报道的来自西班牙东南部的豌豆分离株聚集在一起，而来自东部地区的另外两个分离株与两个意大利分离株和两个希腊分离株聚集在一起。这两个簇在遗传上是不同的，属于多谱系OBR组。OBR组主要包含来自芸苔属植物和萝卜以外寄主的TuMV分离株，且大多源自地中海国家。这些新序列为OBR组提供了进一步的系统发育解析。尽管在西班牙发现了新的TuMV分离株，但它们并未与任何严重的疾病爆发相关联。
