Nielsen L L, Pedersen R A
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143.
J Exp Zool. 1991 Jan;257(1):128-33. doi: 10.1002/jez.1402570117.
A reporter gene construct containing the Drosophila alcohol dehydrogenase (ADH) gene under regulation of the Rous sarcoma virus long terminal repeat (RSV LTR) was microinjected into mouse zygote pronuclei. ADH activity after injection of the RSV-ADH construct was visualized in cultured embryos by means of a simple histochemical enzyme assay. The RSV LTR was an efficient promoter that led to abundant ADH activity at all stages of preimplantation development. There was a high incidence of mosaicism among stained embryos.
将一个含有在劳氏肉瘤病毒长末端重复序列（RSV LTR）调控下的果蝇乙醇脱氢酶（ADH）基因的报告基因构建体显微注射到小鼠受精卵原核中。通过一种简单的组织化学酶测定法，在培养的胚胎中观察到注射RSV - ADH构建体后的ADH活性。RSV LTR是一个有效的启动子，在植入前发育的所有阶段都能导致丰富的ADH活性。在染色的胚胎中嵌合体的发生率很高。
