Santo Antonio S, Cunningham Ariana M, Alhassan Sofiya, Browne Richard W, Burton Harold, Leddy John J, Grandjean Peter W, Horvath Steven M, Horvath Peter J
Division of Healthful Living and Sports Studies, School of Health Sciences, Lenoir-Rhyne College, Physiology Laboratory, Hickory, NC 28603, USA.
Appl Physiol Nutr Metab. 2008 Jun;33(3):489-500. doi: 10.1139/H08-023.
The traditional lipid profile compared with nuclear magnetic resonance (NMR) may underestimate the risk for cardiovascular disease and may explain some of the discrepancies in results between studies analyzing the salubrious effects of soy. Our purpose was to compare the traditional lipid profile with NMR quantification of the number of lipoprotein particles, subclasses, and diameters or sizes in 30 sedentary males, between 18 and 30 years of age, consuming 1 of the following 3 supplements daily for 28 days: milk protein (Milk), isoflavone-poor soy protein (Soy-), or isoflavone-rich soy protein (Soy+). The study used a double-blind, parallel-arm design with random assignment to 1 of the 3 protein supplement groups. Fasting EDTA blood samples were collected at baseline and after 28 days of supplementation and analyzed for the number and size of very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) particles, respectively. Fasting serum samples were analyzed for concentrations of total cholesterol (TC), LDL cholesterol (LDL-C), total HDL cholesterol (HDL-C), HDL(2)-C, HDL(3)-C, triglycerides (TGs), free fatty acids (FFAs), and glucose. Fasting heparin blood samples were collected at baseline and after supplementation and analyzed for apolipoproteins A-I, A-II, B, C-II, C-III, and E, as well as hepatic and lipoprotein lipase concentrations. HDL3-C increased by 47.2% after Soy+ supplementation and hepatic lipase decreased 19.2% after Soy- supplementation (p < 0.05). HDL-C and apolipoproteins A-I and A-II were found to increase in all 3 groups (p < 0.05). Results support that NMR analysis of lipoprotein particle number and size are not more sensitive to the effect of soy protein on CVD risk compared with the traditional lipid profile. Furthermore, the lack of isoflavones in soy protein seems to have a deleterious effect on hepatic lipase.
与核磁共振（NMR）相比，传统血脂谱可能会低估心血管疾病风险，这或许可以解释在分析大豆有益作用的研究之间，结果存在差异的部分原因。我们的目的是比较传统血脂谱与NMR对30名年龄在18至30岁之间的久坐男性脂蛋白颗粒数量、亚类以及直径或大小的量化结果。这些男性连续28天每天服用以下3种补充剂中的一种：乳蛋白（牛奶）、异黄酮含量低的大豆蛋白（大豆 -）或异黄酮含量高的大豆蛋白（大豆 +）。该研究采用双盲、平行组设计，随机分配至3种蛋白质补充剂组中的一组。在基线期和补充28天后采集空腹乙二胺四乙酸（EDTA）血样，并分别分析极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）颗粒的数量和大小。对空腹血清样本分析总胆固醇（TC）、低密度脂蛋白胆固醇（LDL - C）、总高密度脂蛋白胆固醇（HDL - C）、HDL(2)-C、HDL(3)-C、甘油三酯（TGs）、游离脂肪酸（FFAs）和葡萄糖的浓度。在基线期和补充后采集空腹肝素血样，并分析载脂蛋白A - I、A - II、B、C - II、C - III和E以及肝脂酶和脂蛋白脂酶浓度。补充大豆 + 后HDL3 - C增加了47.2%，补充大豆 - 后肝脂酶降低了19.2%（p < 0.05）。在所有3组中均发现HDL - C以及载脂蛋白A - I和A - II增加（p < 0.05）。结果表明，与传统血脂谱相比，NMR对脂蛋白颗粒数量和大小的分析对大豆蛋白对心血管疾病风险的影响并不更敏感。此外，大豆蛋白中缺乏异黄酮似乎对肝脂酶有有害影响。
