铜绿假单胞菌转座子诱导的细胞毒素缺陷型突变体的分离与鉴定
Isolation and characterization of a transposon-induced cytotoxin-deficient mutant of Pseudomonas aeruginosa.
作者信息
Bopp L H, Baltch A L, Hammer M C, Franke M A, Smith R P, Lutz F
机构信息
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
出版信息
Infect Immun. 1991 Feb;59(2):708-11. doi: 10.1128/iai.59.2.708-711.1991.
In order to provide a better system for investigating the role of cytotoxin in pathogenesis, we mutated wild-type Pseudomonas aeruginosa PA158 by introducing a transposon. The resulting pool of mutants was screened for cytotoxin-deficient strains. One mutant strain, PA114F5, was compared with PA158. Except for cytotoxin production and antibiotic resistance (specified by the transposon), the two strains appear isogenic. This mutant strain should be useful in further clarifying the role of cytotoxin in pathogenesis.
为了提供一个更好的系统来研究细胞毒素在发病机制中的作用，我们通过引入转座子对野生型铜绿假单胞菌PA158进行了突变。对产生的突变体库进行筛选，以寻找细胞毒素缺陷菌株。将其中一个突变菌株PA114F5与PA158进行比较。除了细胞毒素产生和抗生素抗性（由转座子决定）外，这两个菌株看起来是同基因的。该突变菌株应有助于进一步阐明细胞毒素在发病机制中的作用。
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