视神经脊髓炎及通过酶联免疫吸附测定法检测的抗水通道蛋白4抗体。

Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay.

作者信息

Hayakawa Sei, Mori Masahiro, Okuta Akiko, Kamegawa Akiko, Fujiyoshi Yoshinori, Yoshiyama Yasumasa, Mitsuoka Kaoru, Ishibashi Kenichi, Sasaki Sei, Hattori Takamichi, Kuwabara Satoshi

机构信息

Department of Neurology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chou-ku, Chiba 260-8670, Japan.

出版信息

J Neuroimmunol. 2008 May 30;196(1-2):181-7. doi: 10.1016/j.jneuroim.2008.03.009. Epub 2008 May 6.

DOI:10.1016/j.jneuroim.2008.03.009

PMID:18462810

Abstract

NMO-IgG, a disease-specific autoantibody for neuromyelitis optica, recognizes aquaporin-4 (AQP4) and has been examined by indirect immunofluorescence assay. We developed an enzyme-linked immunosorbent assay (ELISA) to detect anti-AQP4 antibodies by establishing methods for expression in a baculovirus system and purification of recombinant AQP4 as antigen. Elevated anti-AQP4 antibody titers in serum were found in 15 (71%) of 21 patients with neuromyelitis optica, 4.3% of 46 patients with multiple sclerosis, none of 51 normal controls, and 2.6% of 115 patients with other neurological diseases. The ELISA system can be substituted for the conventional NMO-IgG assay.

摘要

视神经脊髓炎特异性自身抗体NMO-IgG可识别水通道蛋白4（AQP4），并已通过间接免疫荧光法进行检测。我们通过建立杆状病毒系统表达方法和重组AQP4作为抗原的纯化方法，开发了一种酶联免疫吸附测定（ELISA）来检测抗AQP4抗体。在21例视神经脊髓炎患者中，有15例（71%）血清抗AQP4抗体滴度升高；46例多发性硬化症患者中有4.3%升高；51名正常对照者中无一例升高；115例其他神经系统疾病患者中有2.6%升高。ELISA系统可替代传统的NMO-IgG检测方法。