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通过原位杂交技术对羊茅属及其相关物种的核糖体 DNA 位点进行物理作图。

Physical mapping of ribosomal DNA sites in Festuca arundinacea and related species by in situ hybridization.

出版信息

Genome. 1997 Jun;40(3):406-10. doi: 10.1139/g97-054.

Abstract

UNLABELLED

The positions of the 18S-5.8S-26S and 5S rRNA genes have been physically mapped on the chromosomes of diploid, tetraploid, and hexaploid Festuca species by in situ hybridization. The number and position of the rDNA sites in the species were compared. The results confirm some of the earlier phylogenetic studies of these species but suggest that some structural rearrangements have occurred and that sites have been lost during polyploidization.

KEYWORDS

Festuca, in situ hybridization, phylogeny, physical mapping, rDNA.

摘要

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通过原位杂交,将 18S-5.8S-26S 和 5S rRNA 基因的位置在二倍体、四倍体和六倍体羊茅属物种的染色体上进行了物理作图。比较了物种中 rDNA 位点的数量和位置。结果证实了这些物种早期的一些系统发育研究,但表明在多倍化过程中发生了一些结构重排,并且某些位点已经丢失。

关键词

羊茅属、原位杂交、系统发育、物理作图、rDNA。

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