用氧化氘取代氧化氢对二十六烷基磷脂酰胆碱叉指状凝胶相和波纹相之间热致转变的影响。

Effect of substitution of hydrogen oxide by deuterium oxide on thermotropic transition between the interdigitated gel phase and the ripple phase of dihexadecylphosphatidylcholine.

作者信息

Ohki K

机构信息

Department of Applied Physics, School of Engineering, Nagoya University, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Jan 15;174(1):102-6. doi: 10.1016/0006-291x(91)90491-o.

DOI:10.1016/0006-291x(91)90491-o

PMID:1846533

Abstract

Thermotropic transitions of dihexadecylphosphatidylcholine (DHPC) dispersions in hydrogen oxide (1H2O) and deuterium oxide (2H2O) were investigated by differential scanning calorimetry (DSC). In DHPC dispersions, transition temperature between interdigitated gel phase (L beta I) and ripple phase (P beta') is lower in 2H2O than in 1H2O, and transition between the ripple phase (P beta') and fluid phase (L alpha) in 2H2O occurs at a temperature slightly higher than in 1H2O. In dipalmitoylphosphatidylcholine (DPPC) dispersions, on the other hand, transition temperature between lamellar gel phase (L beta') and ripple phase is higher in 2H2O than in 1H2O. These results suggest that the interdigitated gel phase is more stable in 1H2O than in 2H2O. To account for the shift of transition temperature by the water substitution, difference of interfacial energies between these aqueous environments is discussed.

摘要

通过差示扫描量热法（DSC）研究了二己基磷脂酰胆碱（DHPC）在氧化氢（H₂O）和氧化氘（D₂O）中的热致转变。在DHPC分散体系中，叉指状凝胶相（LβI）和波纹相（Pβ'）之间的转变温度在D₂O中比在H₂O中更低，并且在D₂O中波纹相（Pβ'）和流体相（Lα）之间的转变发生温度略高于在H₂O中的情况。另一方面，在二棕榈酰磷脂酰胆碱（DPPC）分散体系中，层状凝胶相（Lβ'）和波纹相之间的转变温度在D₂O中比在H₂O中更高。这些结果表明叉指状凝胶相在H₂O中比在D₂O中更稳定。为了解释水替换导致的转变温度变化，讨论了这些水性环境之间界面能的差异。