一种基于DNA双链体的、为卟啉衍生物量身定制的荧光传感器。

A DNA duplex-based, tailor-made fluorescent sensor for porphyrin derivatives.

作者信息

Fujimoto Kazuhisa, Muto Yu, Inouye Masahiko

机构信息

Graduate School of Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan.

出版信息

Bioconjug Chem. 2008 Jun;19(6):1132-4. doi: 10.1021/bc800133e. Epub 2008 May 9.

DOI:10.1021/bc800133e

PMID:18465890

Abstract

We synthesized a DNA-based fluorescent sensor, tailored to the recognition and signaling for hydrophobic guest molecules. The sensor molecule is made of three functional moieties, per-O-methylated beta-cyclodextrin (per-Me-beta-CD), DNA, and pyrene as guest-binding, dimerizing, and signaling ones, respectively. Addition of a porphyrin derivative as a hydrophobic guest molecule induced emission switching of the sensor molecule from monomer to excimer due to the DNA hybridization promoted by the host-guest binding.

摘要

我们合成了一种基于DNA的荧光传感器，该传感器专为识别疏水性客体分子并发出信号而设计。传感器分子由三个功能部分组成，分别是全-O-甲基化β-环糊精（全-Me-β-CD）、DNA和芘，它们分别作为客体结合、二聚化和信号传导部分。加入卟啉衍生物作为疏水性客体分子后，由于主客体结合促进了DNA杂交，传感器分子的发射从单体转变为激基缔合物。