纳米乳剂包裹的MHS疫苗的抗肿瘤免疫反应
[Anti-tumor immune responses of nanoemulsion-encapsulated MHS vaccine].
作者信息
Ge Wei, Sun Yu-jing, Li Yuan, Zhang Shan-hong, Zhang Xiu-min, Huang Yang, Hu Pei-zhen, Sui Yan-fang
机构信息
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 May;24(5):457-60.
AIM
To prepare nanoemulsion-encapsulated MAGE1-Hsp70/SEA and to evaluate its anti-tumor effects in mouse.
METHODS
Nanoemulsion vaccine NE(MHS) was prepared using magnetic ultrasound methods and used to immunize C57BL/6 mice. The cellular immune responses were detected by IFN-gamma ELISPOT and LDH release assay. The tumor challenge assay was performed too.
RESULTS
(1) The mean size of NE(MHS) was (20+/-5) nm. The encapsulation rate was 87% and the nanoemulsion vaccine had a good stability. (2) The frequency of MAGE-1 specific CTL and cytotoxicity of CTL to B16-MAGE-1 cells were both greatly enhanced in immunization group than those in control group (P<0.05). NE(MHS) could significantly delay the appearance of tumors and increase the percentage of tumor-free mice.
CONCLUSION
The nanoemulsion had excellent physical and chemical characteristics. It could elicit MAGE-1-specific cellular immune response and anti-tumor effects against the MAGE-1-expressing tumor.
目的
制备纳米乳剂包裹的MAGE1-Hsp70/SEA，并评估其对小鼠的抗肿瘤作用。
方法
采用磁超声法制备纳米乳剂疫苗NE(MHS)，并用于免疫C57BL/6小鼠。通过IFN-γ ELISPOT和LDH释放试验检测细胞免疫反应。同时进行肿瘤攻击试验。
结果
(1)NE(MHS)的平均粒径为(20±5)nm。包封率为87%，纳米乳剂疫苗具有良好的稳定性。(2)免疫组中MAGE-1特异性CTL的频率和CTL对B16-MAGE-1细胞的细胞毒性均显著高于对照组(P<0.05)。NE(MHS)可显著延迟肿瘤的出现并增加无瘤小鼠的百分比。
结论
该纳米乳剂具有优异的物理化学特性。它能引发MAGE-1特异性细胞免疫反应，并对表达MAGE-1的肿瘤产生抗肿瘤作用。
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