Williams Lisa K, McMeechan Alisdair, Baalham Tamsin, Ward Laura, Humphrey Tom J, Jørgensen Frieda
Foodborne Zoonoses Unit, Health Protection Agency, School of Clinical Veterinary Science, Division of Veterinary Pathology Infection and Immunity, University of Bristol, Langford, Bristol BS40 5DU, UK.
J Food Prot. 2008 Apr;71(4):835-8. doi: 10.4315/0362-028x-71.4.835.
In this study, the conventional International Organization for Standardization (ISO) culture method was compared with the DuPont Qualicon BAX system, a high-throughput, rapid molecular assay that can be used to detect several bacterial species, including Campylobacter jejuni and Campylobacter coli in diverse sample types. Standard enrichment culture is a time-consuming process, taking up to 6 days to obtain a confirmed result. Rapid molecular assays have been developed that provide results within 24 h. Naturally contaminated samples from the poultry production chain were examined for the presence of Campylobacter spp. Samples from broiler chicken ceca (n = 100), fresh chicken carcass rinses (n = 60), and bootsocks (gauze sock walked through a broiler chicken house; n = 50) were enriched according to the ISO 10272 method in Bolton broth specifically designed to detect Campylobacter spp. in complex sample types. Samples were enriched without blood for use with the BAX system using the Campylobacter BAX kits for the detection of C. jejuni and C. coli. Samples also were directly plated onto modified charcoal cefperazone deoxycholate agar, and results were compared with those from the enriched samples for the ability to detect Campylobacter spp. Campylobacter spp. were isolated from 49% of samples with conventional enrichment cultures, from 48% with direct culture, from 68% with the BAX system and enrichment cultures, and from 62% with the BAX system used directly with samples. Overall, the BAX system detected more positive samples than did the conventional culture method and is an effective methodology for the rapid and reliable detection of Campylobacter spp. from diverse sample types.
在本研究中，将传统的国际标准化组织（ISO）培养方法与杜邦Qualicon BAX系统进行了比较，后者是一种高通量、快速分子检测方法，可用于检测多种细菌物种，包括不同样本类型中的空肠弯曲菌和结肠弯曲菌。标准富集培养是一个耗时的过程，需要长达6天才能获得确诊结果。已开发出快速分子检测方法，可在24小时内提供结果。对来自家禽生产链的天然污染样本进行了弯曲菌属检测。按照ISO 10272方法，将来自肉鸡盲肠（n = 100）、新鲜鸡胴体冲洗液（n = 60）和靴袜（穿过肉鸡舍的纱布袜；n = 50）的样本在专门设计用于检测复杂样本类型中弯曲菌属的博尔顿肉汤中进行富集。使用用于检测空肠弯曲菌和结肠弯曲菌的弯曲菌BAX试剂盒，在无血液的情况下对样本进行富集，以便与BAX系统配合使用。样本还直接接种到改良的木炭头孢哌酮脱氧胆酸盐琼脂上，并将结果与富集样本检测弯曲菌属的能力进行比较。通过传统富集培养，从49%的样本中分离出弯曲菌属；通过直接培养，从48%的样本中分离出弯曲菌属；通过BAX系统和富集培养，从68%的样本中分离出弯曲菌属；通过直接使用BAX系统检测样本，从62%的样本中分离出弯曲菌属。总体而言，BAX系统检测到的阳性样本比传统培养方法更多，是一种从不同样本类型中快速可靠地检测弯曲菌属的有效方法。
