Mimura Haruo, Sato Ryusei, Furuyama Yuichi, Taniike Akira, Yagi Masahiro, Yoshida Kazutoshi, Kitamura Akira
Graduate School of Maritime Sciences, Kobe University, 5-1-1, Fukae, Higashinada, Kobe 658-0022, Japan.
Mar Pollut Bull. 2008;57(6-12):877-82. doi: 10.1016/j.marpolbul.2008.03.029. Epub 2008 May 12.
The isolate, Pesudoalteromonas sp. TBT1, could grow to overcome the toxicity of tributyltin chloride (TBTCl) up to 30 microM in the absence of Cl(-) in the medium until the cells reached an exponential phase of growth. The viability, however, was reduced after the cells reached a stationary phase. The degradation products, such as dibutyltin (DBT) and monobutyltin (MBT), were not detected in the growth medium, indicating that the isolate has no ability to degrade TBT into less toxic DBT and MBT. Up to about 10(7.5) TBT molecules were adsorbed by a single cell. The observation of morphological changes with an electron microscope showed that the cell surface became wrinkled after exposure to the lethal concentration of 10 mM TBTCl. These results indicate that the resistance of the isolate toward the toxicity of TBTCl is not related to the unique cell surface, which seems to play an important role in preventing the diffusion of TBTCl into the cytoplasm.
分离菌株假交替单胞菌属（Pesudoalteromonas sp.）TBT1在培养基中无Cl(-)的情况下，能够生长以克服高达30微摩尔的三丁基氯化锡（TBTCl）的毒性，直至细胞达到指数生长期。然而，在细胞进入稳定期后，活力会降低。在生长培养基中未检测到降解产物，如二丁基锡（DBT）和单丁基锡（MBT），这表明该分离菌株没有能力将TBT降解为毒性较小的DBT和MBT。单个细胞最多可吸附约10(7.5)个TBT分子。用电子显微镜观察形态变化表明，在暴露于10 mM TBTCl的致死浓度后，细胞表面变得皱缩。这些结果表明，该分离菌株对TBTCl毒性的抗性与独特的细胞表面无关，而细胞表面似乎在防止TBTCl扩散到细胞质中起着重要作用。
