利用低温喷雾电离-傅里叶变换离子回旋共振质谱法检测微囊藻毒素-金属络合物

Detection of microcystin-metal complexes by using cryospray ionization-Fourier transform ion cyclotron resonance mass spectrometry.

作者信息

Saito Kazunori, Sei Yoshihisa, Miki Shinichi, Yamaguchi Kentaro

机构信息

Bruker Daltonics K.K., 3-9 A-6F Moriya-cho, Kanagawa-ku, Yokohama, Kanagawa, Japan.

出版信息

Toxicon. 2008 Jun 15;51(8):1496-8. doi: 10.1016/j.toxicon.2008.03.026. Epub 2008 Mar 28.

DOI:10.1016/j.toxicon.2008.03.026

PMID:18468650

Abstract

Complexes of microcystins (MCs) and metal ions were detected with mass spectrometry. To observe the MCs-metals complexes, CryoSpray ionization ion source was developed and it was equipped on a commercial Fourier transform ion resonance mass spectrometer. Mixtures of two MCs and five metals were applied to Fourier transform ion cyclotron resonance mass spectrometry coupled with CryoSpray. The analyses showed complexes of MC-LR-Fe(II), -Zn, -Cu, -Mg, MC-RR-Fe(II), -Zn, -Cu, and -Mg but MC-LR-Fe(III) and MC-RR-Fe(III) were not observed. Present study suggested that MCs may play roles in metal ion uptake and/or accumulation in the algal cells.

摘要

通过质谱法检测了微囊藻毒素（MCs）与金属离子的复合物。为了观察MCs-金属复合物，开发了低温喷雾电离离子源，并将其配备在商用傅里叶变换离子共振质谱仪上。将两种MCs和五种金属的混合物应用于与低温喷雾联用的傅里叶变换离子回旋共振质谱仪。分析结果显示存在MC-LR-Fe(II)、-Zn、-Cu、-Mg、MC-RR-Fe(II)、-Zn、-Cu和-Mg的复合物，但未观察到MC-LR-Fe(III)和MC-RR-Fe(III)。目前的研究表明，MCs可能在藻类细胞对金属离子的摄取和/或积累中发挥作用。