Payne Anne H, Glish Gary L
Department of Chemistry, University of North Carolina, Chapel Hill, NC, USA.
Methods Enzymol. 2005;402:109-48. doi: 10.1016/S0076-6879(05)02004-5.
Instruments that trap ions in a magnetic and/or electric field play a very important role in the analysis of biomolecules. The two predominant instruments in the category of trapping instrument are the quadrupole ion trap mass spectrometer (QIT-MS) and the ion cyclotron resonance (ICR) MS. The latter is also commonly called Fourier transform MS (FT-MS). The QIT is an inexpensive, simple, and rugged MS used for various routine applications. The ICR-MS is an expensive, high-performance instrument with figures of merit for resolution and mass accuracy surpassing all other mass spectrometers. This chapter covers the basic principles of operation of these instruments, including the trapping/manipulation/detection of ions and various approaches used to activate ions to perform tandem mass spectrometry experiments.
在磁场和/或电场中捕获离子的仪器在生物分子分析中起着非常重要的作用。捕获仪器类别中两种主要的仪器是四极杆离子阱质谱仪（QIT-MS）和离子回旋共振（ICR）质谱仪。后者通常也称为傅里叶变换质谱仪（FT-MS）。QIT是一种用于各种常规应用的廉价、简单且坚固耐用的质谱仪。ICR-MS是一种昂贵的高性能仪器，其分辨率和质量准确度的品质因数超过所有其他质谱仪。本章涵盖了这些仪器的基本操作原理，包括离子的捕获/操纵/检测以及用于激活离子以进行串联质谱实验的各种方法。
