1型糖尿病年轻患者对氧磷酶-1基因p.Leu54Met多态性与足底筋膜厚度之间的关联。

Association between p.Leu54Met polymorphism at the paraoxonase-1 gene and plantar fascia thickness in young subjects with type 1 diabetes.

作者信息

Gallego Patricia H, Craig Maria E, Duffin Anthony C, Bennetts Bruce, Jenkins Alicia J, Hofer Sabine, Lam Albert, Donaghue Kim C

机构信息

Institute of Endocrinology and Diabetes, The Children's Hospital at Westmead, Sydney, New South Wales, Australia.

出版信息

Diabetes Care. 2008 Aug;31(8):1585-9. doi: 10.2337/dc07-2236. Epub 2008 May 9.

DOI:10.2337/dc07-2236

PMID:18469206

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2494642/

Abstract

OBJECTIVE

In type 1 diabetes, plantar fascia, a collagen-rich tissue, is susceptible to glycation and oxidation. Paraoxonase-1 (PON1) is an HDL-bound antioxidant enzyme. PON1 polymorphisms have been associated with susceptibility to macro- and microvascular complications. We investigated the relationship between plantar fascia thickness (PFT) and PON1 gene variants, p.Leu54Met, p.Gln192Arg, and c.-107C>T, in type 1 diabetes.

RESEARCH DESIGN AND METHODS

This was a cross-sectional study of 331 adolescents with type 1 diabetes (162 male and 169 female). PFT was assessed by ultrasound, PON1 was assessed by genotyping with PCR and restriction fragment-length polymorphism, and serum PON1 activity was assessed by rates of hydrolysis of paraoxon and phenylacetate.

RESULTS

Median (interquartile range) age was 15.4 (13.5-17.3) years, and diabetes duration was 7.6 (4.9-10.6) years. The distribution of p.Leu54Met genotypes was LL 135 (40.8%), ML 149 (45%), and MM 47 (14.2%). PFT was abnormal (>1.7 mm) in 159 adolescents (48%). In multivariate analysis, predictors of abnormal PFT were ML/LL versus MM p.Leu54Met polymorphism (odds ratio 3.84 [95% CI 1.49-9.82], P = 0.005); BMI (percentile) (1.02 [1.01-1.03], P = 0.007); systolic blood pressure (percentile) (1.01 [1.00-1.02], P = 0.03); and male sex (3.29 [1.98-5.46], P < 0.001).

CONCLUSIONS

Thickening of the plantar aponeurosis occurs predominantly in overweight and male adolescents with type 1 diabetes. The MM genotype at PON1 p.Leu54Met is associated with a reduced risk of abnormal PFT.

摘要

目的

在1型糖尿病中，富含胶原蛋白的足底筋膜易发生糖基化和氧化。对氧磷酶-1（PON1）是一种与高密度脂蛋白结合的抗氧化酶。PON1基因多态性与大血管和微血管并发症的易感性有关。我们研究了1型糖尿病患者足底筋膜厚度（PFT）与PON1基因变异p.Leu54Met、p.Gln192Arg和c.-107C>T之间的关系。

研究设计与方法

这是一项对331名1型糖尿病青少年（162名男性和169名女性）的横断面研究。通过超声评估PFT，通过聚合酶链反应和限制性片段长度多态性基因分型评估PON1，并通过对氧磷和苯乙酸的水解速率评估血清PON1活性。

结果

中位（四分位间距）年龄为15.4（13.5 - 17.3）岁，糖尿病病程为7.6（4.9 - 10.6）年。p.Leu54Met基因型的分布为LL 135例（40.8%），ML 149例（45%），MM 47例（14.2%）。159名青少年（48%）的PFT异常（>1.7 mm）。在多变量分析中，PFT异常的预测因素为ML/LL与MM的p.Leu54Met多态性（比值比3.84 [95%可信区间1.49 - 9.82]，P = 0.005）；体重指数（百分位数）（1.02 [1.01 - 1.03]，P = 0.007）；收缩压（百分位数）（1.01 [1.00 - 1.02]，P = 0.03）；以及男性性别（3.29 [1.98 - 5.46]，P < 0.001）。