铜离子和汞离子对人体红细胞作用的自旋标记研究。

A spin label study of the action of cupric and mercuric ions on human red blood cells.

作者信息

Gwozdziński K

机构信息

Department of Biophysics, University of Lódz, Poland.

出版信息

Toxicology. 1991 Jan;65(3):315-23. doi: 10.1016/0300-483x(91)90090-n.

DOI:10.1016/0300-483x(91)90090-n

PMID:1846987

Abstract

The influence of heavy metal ions on human red blood cells was studied. Electron spin resonance studies of human erythrocytes labelled either with methyl 5-doxylpalmitate or methyl 12-doxylsterate indicated that cupric and mercuric ions increase the rigidity of membrane lipid bilayers. Both metals also induce conformational changes of membrane proteins and modify cell internal peptides and proteins as indicated by a 4-maleimido-2,2,6,6-tetramethylpiperidine-1-oxyl label ESR study. Copper and mercury ions decreased internal viscosity of red blood cells.

摘要

研究了重金属离子对人体红细胞的影响。对用5-棕榈酰氧基甲基或12-硬脂酰氧基甲基标记的人体红细胞进行电子自旋共振研究表明，铜离子和汞离子会增加膜脂双层的刚性。如4-马来酰亚胺基-2,2,6,6-四甲基哌啶-1-氧基标记的电子自旋共振研究所示，这两种金属还会诱导膜蛋白的构象变化，并改变细胞内的肽和蛋白质。铜离子和汞离子降低了红细胞的内部粘度。