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六倍体普通小麦(Triticum aestivum L. em. Thell.)第 6 组染色体的分子遗传图谱。

Molecular genetic maps of the group 6 chromosomes of hexaploid wheat (Triticum aestivum L. em. Thell.).

出版信息

Genome. 1996 Apr;39(2):359-66. doi: 10.1139/g96-046.

Abstract

Restriction fragment length polymorphism (RFLP) maps of chromosomes 6A, 6B, and 6D of hexaploid wheat (Triticum aestivum L. em. Thell.) have been produced. They were constructed using a population of F7-8 recombinant inbred lines derived from a synthetic wheat x bread wheat cross. The maps consist of 74 markers assigned to map positions at a LOD >= 3 (29 markers assigned to 6A, 24 to 6B, and 21 to 6D) and 2 markers assigned to 6D ordered at a LOD of 2.7. Another 78 markers were assigned to intervals on the maps. The maps of 6A, 6B, and 6D span 178, 132, and 206 cM, respectively. Twenty-one clones detected orthologous loci in two homoeologues and 3 detected an orthologous locus in each chromosome. Orthologous loci are located at intervals of from 1.5 to 26 cM throughout 70% of the length of the linkage maps. Within this portion of the maps, colinearity (homosequentiality) among the three homoeologues is strongly indicated. The remainder of the linkage maps consists of three segments ranging in length from 47 to 60 cM. Colinearity among these chromosomes and other Triticeae homoeologous group 6 chromosomes is indicated and a consensus RFLP map derived from maps of the homoeologous group 6 chromosomes of hexaploid wheat, tetraploid wheat, Triticum tauschii, and barley is presented. Key words : RFLP, wheat, linkage maps, molecular markers.

摘要

已构建出六倍体普通小麦(Triticum aestivum L. em. Thell.)第 6A、6B 和 6D 染色体的限制性片段长度多态性(RFLP)图谱。这些图谱是利用源自合成小麦与面包小麦杂交的 F7-8 重组自交系群体构建的。图谱由 74 个标记组成,这些标记被分配到 LOD >= 3 的图谱位置(29 个标记分配到 6A,24 个标记分配到 6B,21 个标记分配到 6D),2 个标记分配到 LOD 为 2.7 的 6D 有序位置。另有 78 个标记被分配到图谱区间。6A、6B 和 6D 图谱分别覆盖 178、132 和 206 cM。21 个克隆在两个同源物中检测到了直系同源基因座,3 个克隆在每条染色体中检测到了一个直系同源基因座。直系同源基因座位于连锁图谱长度的 70%的间隔内,从 1.5 到 26 cM。在图谱的这一部分,三个同源物之间的共线性(同线性)得到了强烈的指示。连锁图谱的其余部分由三个长度从 47 到 60 cM 的片段组成。这些染色体之间的共线性和其他六倍体小麦、四倍体小麦、Triticum tauschii 和大麦的同源群 6 染色体之间的共线性得到了指示,并呈现了来自六倍体小麦、四倍体小麦、Triticum tauschii 和大麦同源群 6 染色体图谱的共识 RFLP 图谱。关键词:RFLP、小麦、连锁图谱、分子标记。

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