Abrunhosa F A, Santiago A P, Abrunhosa J P
Núcleo de Estudos Costeiros, Campus de Bragança, Universidade Federal do Pará, Bragança, PA, 68600-000, Brazil.
Braz J Biol. 2008 Feb;68(1):179-86. doi: 10.1590/s1519-69842008000100026.
The early stages of the Panulirus echinatus were hatched and reared in the laboratory. Ovigerous females were captured in their habitat and carefully transported to the laboratory. Larvae were transferred in a recirculation water tank at a density of 10 larvae.L(-1). The larvae were fed on Artemia and gonads of mussel Brachydonts sp. Microalgae Dunaliella viridis was added at a concentration of 150 x 10(4) cell.mL(-1). Larvae and exuviae of each zoeal stage were preserved in an alcohol 70% + glycerin (1:1) solution. The phyllosomas moulted eight times; the intermoulting period of each instar averaged about 7 to 10 days. The main morphological changes of each appendage were described in detail, illustrated and compared with previous reports.
棘刺龙虾的早期阶段在实验室中孵化和饲养。怀卵雌虾在其栖息地捕获,并小心运至实验室。幼虫以每升10只的密度转移到循环水箱中。幼虫以卤虫和贻贝Brachydonts sp.的性腺为食。添加浓度为150×10⁴个细胞·毫升⁻¹的微藻杜氏盐藻。每个蚤状幼体阶段的幼虫和蜕皮保存在70%酒精+甘油(1:1)溶液中。叶状幼体蜕皮八次;每个龄期的蜕皮间期平均约为7至10天。详细描述了每个附肢的主要形态变化,进行了图示,并与先前的报告进行了比较。