Nolan R D, Lapetina E G
Division of Cell Biology, Burroughs Wellcome Co., Research Triangle Park, NC 27709.
Biochem Biophys Res Commun. 1991 Jan 31;174(2):524-8. doi: 10.1016/0006-291x(91)91448-l.
Untreated human platelets labeled to equilibrium with 32Pi contained undetectable levels of 3-phosphorylated phosphoinositides. Stimulation of platelets with thrombin for 5 min resulted in an enormous increase in the amount of phosphatidylinositol 3,4-bisphosphate (Nolan and Lapetina, J. Biol. Chem. 265, 2441-2445; 1990). We now report that the levels of phosphatidylinositol 3,4,5-trisphosphate are greatly elevated within 90 s of treatment of platelets with thrombin. Phosphatidylinositol 3,4,5-phosphate might have an important role in platelet aggregation.
用³²Pi标记至平衡的未处理人血小板中3 - 磷酸化磷酸肌醇水平无法检测到。用凝血酶刺激血小板5分钟导致磷脂酰肌醇3,4 - 二磷酸的量大幅增加（诺兰和拉佩蒂纳，《生物化学杂志》265卷，2441 - 2445页；1990年）。我们现在报告，在用凝血酶处理血小板90秒内，磷脂酰肌醇3,4,5 - 三磷酸的水平大幅升高。磷脂酰肌醇3,4,5 - 磷酸可能在血小板聚集中起重要作用。
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