Evan A P, Dail W G, Dammrose D, Palmer C
Anat Rec. 1976 Aug;185(4):433-45. doi: 10.1002/ar.1091850405.
The application of scanning electron microscopy to the study of cell surfaces is limited in intact tissues, because extracellular material may often obscure the details of nonluminal surfaces. To remove connective tissue elements we have treated human skin and both kidney, and an autonomic ganglion of the rat with hydrochloric acid and collagenase. Regional variations in the basal surface of the nephron are noted following removal of the basement membrane. The basilar interdigitations of the cells of the proximal tubule appeared as parallel ridges encircling the tubule. Ridges on the parietal epithelium of Bowman's capsule were randomly arranged and alternated with smooth surfaces. The dermal surface of the human epidermis has an alveolar or honeycomb appearance due to the elevation of the epidermal ridges and numerous pits for the dermal pegs. At higher magnifications the basal surface of cells of the stratum germinativum possessed numerous and irregular projections. Neurons with their processes are evident in the autonomic ganglion. The soma of the neurons are enclosed by flattened satellite cells. Irregular spaces between opposed satellite cells are interpreted as regions for the passage of processes related to the ganglion cells. Nodes of Ranvier were clearly seen along nerve fibers. Some pitting of the nerve fibers was also noted. The HCl-collagenase method has the advantage of the removal of collagen and basement membrane while preserving the structural integrity of the cell surface.
扫描电子显微镜在完整组织的细胞表面研究中的应用受到限制，因为细胞外物质常常会掩盖非管腔表面的细节。为了去除结缔组织成分，我们用盐酸和胶原酶处理了人类皮肤、大鼠的肾脏以及一个自主神经节。去除基底膜后，观察到肾单位基底表面的区域差异。近端小管细胞的基底指状突起呈现为环绕小管的平行嵴。鲍曼囊壁层上皮的嵴随机排列，与光滑表面交替出现。由于表皮嵴的隆起和真皮乳头的众多凹坑，人类表皮的真皮表面呈现出肺泡状或蜂窝状外观。在更高放大倍数下，生发层细胞的基底表面有许多不规则的突起。自主神经节中可见带有突起的神经元。神经元的胞体被扁平的卫星细胞包围。相对卫星细胞之间的不规则间隙被解释为与神经节细胞相关的突起通过的区域。沿神经纤维可清楚地看到郎飞结。还注意到一些神经纤维有凹陷。盐酸 - 胶原酶法的优点是在保留细胞表面结构完整性的同时去除了胶原蛋白和基底膜。
